白藜芦醇对金黄色葡萄球菌标准株的抑菌作用及机制研究

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1、授予单位代码10089学号或申请号12427HebeiMedicalUniversity硕士学位论文在职科学学位白藜芦醇对金黄色葡萄球菌标准株的抑菌作用及机制研究学位申请人：王義导师：吴淑慧副教授专业：病原生物学二级学院：基础医学院2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师（或指导小组）的指导下，由本人独立完成。本学位论文研究所获得的研究成果,其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用。凡发表与学位论文主要内容相关的论文,第一

2、署名为单位河北医科大学，试验材料、原始数据、申报的专利等知识产权均归河北医科大学所有。否则，承担相应法律责任。研究生签名：王^导师签章级学院领导盖章：To/jr年Z月日河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人已经发表或撰写的研究成果,指导教师对此进行了审定。本论文由本人独立撰写，文责自负。研究生签名：土森导师签章：年<月日目录中文摘要································

3、·············································1英文摘要·············································································5英文缩写·············································································10研究论文白藜芦醇对金黄色葡萄球菌标准株抑菌作用和机制的研究前言········

4、·····································································11材料与方法····································································12结果·············································································16附图·····················

5、························································19附表·············································································24讨论·············································································26结论····························

6、·················································29参考文献·······································································30综述白藜芦醇抑菌作用及抑菌机制研究进展……………………….34致谢···················································································40个人简历

7、·············································································41中文摘要白藜芦醇对金黄色葡萄球菌标准株抑菌作用及机制研究摘要目的：白藜芦醇(Resveratrol，RES)是植物遇真菌侵袭时分泌的一种植物防御成分，属天然多酚类物质。1940年从植物白藜芦根茎里提取并命名为Resveratrol。含RES的植物资源丰富，葡萄藤、虎杖等植物都可加工提纯RES，废弃植物如花生根、葡萄皮等也含有RES。RES具

8、有抗癌、免疫调节、预防心血管疾病、抗氧化、抗病毒等多种生物学活性，对多种微生物也具有抑制作用，但抑菌机制尚不明确。几十年来，临床病原菌耐药性不断增加，尤其是多重耐药的金黄色葡萄球菌让临床医师选择抗生素难上加难，副作用小且价格低廉的中药可作为辅助药物供临床选择。本研究选用金黄色葡萄球菌标准株ATCC25923(简称金葡菌标准株)作为研究主体，通过观察分析RES作用金葡菌标准株后，其最低抑菌浓度、生长曲线、细胞膜通透性、超微结构等指标的变化，研究RES对金葡菌标准株抑菌作用及抑菌机制，为RES进一步