Springer.Advances.in.Biochemical.Engineering.vol.76.Biotechnology.Modern.Adva

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1、PrefacePrefaceDuetotheirversatilityandresolution,chromatographicseparationsofcomplexmixturesofbiologicalsareusedformanypurposesinacademiaandindustry.Ifanything,recentdevelopmentsinthelifescienceshaveincreasedtheinterestandneedforchromatographybeitforqualitycontrol,proteomicsorthedown-streamproces

2、singofthehighvalueproductsofmodernbiotechnology.How-ever,themany“challenges”ofpresentdaychromatographyandespeciallyoftheHPLCofbiomacromoleculessuchasproteins,arealsopresentinthemindofanypractitioner.Infact,someoftheselatterweresuchhindrancesthatmuchresearchwasnecessaryinordertoovercomeandcircumve

3、ntthem.Thisbookintroducesthereadertosomeoftherecentlyproposedsolutions.Capillaryelec-trochromatography(CEC),forexample,thelatestandmostpromisingbranchofanalyticalchromatography,isstillhinderedfromfindingbroaderapplicationbydifficultiesrelatedtosomethingassimpleasthepackingofasuitablecolumn.Thelat

4、estsolutionsforthisbutalsothestateofartofCECingeneralaredis-cussedinthechapterwrittenbyFrantisekSvec.Thedifficultyofcombiningspeed,resolutionandcapacitywhenusingtheclassicalporousbeadtypesta-tionaryphaseshasevenbeencalledthe“dilemmaofproteinchromatography”.Muchprogresshasbeenmadeinthisareabythead

5、ventofmonolithicandrelat-edcontinuousstationaryphases.Thecomplexnatureofmanyofthesamplestobeanalyzedandseparatedinbiochromatographyoftenrequirestheuseofsomehighlyspecific(“affinity”)ligands.Sincetheycanberaisedinaspecificmannertomanybioproducts,proteinligandssuchasantibodieshaveallowedsomeverysel

6、ectivesolutionsinthepast.However,theyalsoareknowntohavesomedis-advantages,includingtheimmunogenicity(toxicity)ofligandscontaminatingthefinalproducts,orthelowstabilityofsuchligands,whichpreventsrepeatedusageoftheexpensivecolumns.Thischallengemaybeovercomeby“molecularimprinting”,atechniques,whichus

7、espurelychemicalmeanstocreatethe“affinity”interaction.Finallyweweremosthappytohavetwoauthorsfromindustryjoinustoreportontheirexperiencewithchromatographyasacontin-uouspreparativeprocess.Readersfromvariousfieldsthuswill