我国流行c基因型野生和耐药hbv稳定复制细胞系的建立及应用

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1、分类号密级UDC保密期限硕士学位论文题目我国流行C基因型野生和耐药HBV稳定复制细胞系的建立及应用作者姓名王琳指导教师徐东平研究员刘妍副研究员培养单位中国人民放军第三0二医院专业名称免疫学论文提交日期2013年06月05日学位授予单位中国人民解放军军事医学科学院答辩委员会主席张叔人教授中国人民解放军军事医学科学院制军事医学科学院研究生学位论文独创性声明秉承军事医学科学院严谨的学风和科研作风，本人声明所呈交的学位论文是本人在导师指导下独立进行的研究工作和取得的研究成果，除了文中特别加以标注和致谢之处外，论文中不包含其他人已经发表或撰写过

2、的研究成果，也不包含为获得军事医学科学院或其它教育机构的学位或证书而使用过的材料。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示了谢意。论文作者签字：签字日期：年月日军事医学科学院保护知识产权声明本学位论文作者完全了解军事医学科学院对研究生在学其间撰写的论文知识产权保护的相关规定。本人撰写的论文是在导师具体指导下，并得到相关研究经费支持下完成的，其数据和研究成果归属于导师和作者本人，知识产权单位属军事医学科学院。本人保证毕业后，以本论文数据和资料发表论文或使用论文工作成果时署名第一单位仍然为军事医学科学院。军

3、事医学科学院有权保留学位论文及其电子版，公布论文的全部或部分内容，可以采用影印、缩印或其他复制手段保存论文、汇编以供查阅和借阅。（保密学位论文在解密后适用本声明）论文作者签字：签字日期：年月日指导教师签字：签字日期：年月日目录缩略词表··············································································1中文摘要······································································

4、··················2英文摘要··················································································5前言·······························································································9正文11第一部分HBV稳定复制细胞株的建立····································11第一节不同的构

5、建策略构建HBV的真核表达载体······································111.材料和方法··············································································112.结果························································································163.讨论······························

6、··································································19第二节稳定复制细胞系的建立····················································211.材料和方法··············································································212.结果············································

7、·································293.讨论·························································································33第二部分HBV稳定复制细胞株的应用··················································351.材料和方法································································

8、······················352.结果··················································································