基于afm的细胞核力学特性原位测试的研究

《基于afm的细胞核力学特性原位测试的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、ắᦻAFMḄ᪶ᱯឋḄẆINSITUQUANTIFICATIONOFLIVINGCELLNUCLEUSMECHANICALPROPERTITYWITHATOMICFORCEMICROSCOPEᏉᜧ2015!6ᨴ#ᑁ%&ᑖ()：TP242.2᪥-ṹ：10213#▭%&ᑖ()：681.5/0：123ắᦻAFMḄ᪶ᱯឋḄẆắẆ4：Ꮙ56:7ᨀᦟᣴᵭ<:3ắ=:>?3ᡠᙠᓫ:>ᵯ3▾EFGH：2015!6ᨴᣴIᓫ：ᜧClassif

2、iedIndex:TP242.2U.D.C:681.5DissertationfortheMasterDegreeinEngineeringINSITUQUANTIFICATIONOFLIVINGCELLNUCLEUSMECHANICALPROPERTITYWITHWITHATOMICFORCEMICROSCOPECandidate:JianminSongSupervisor:Prof.XieHuiAcademicDegreeAppliedfor:MasterofEngineeringSpecialty:MechanicalEnginee

3、ringAffiliation:SchoolofMechatronicsEngineeringDateofDefense:July,2015Degree-Conferring-Institution:HarbinInstituteofTechnologyᜧ3ắᦻ᤺⌕4ᱥḄᱯឋMḄN᪀、QRSTḄ“4ὁVW”YYZᐵ。]^_ᵫabᘤdᡂ，ᐸh᪶ij⍡lᱥmḄn⌕opᘤ，ᙠ᦮rḄ4sᕜHhuᓰᨵxᐵy⌕Ḅiᵨ，Ẇ᪶Ḅᱯឋ{

4、}~4sḄᝍᨵḼy⌕Ḅ。ẆᱯឋḄabh，

5、（AFM)Sᐸ、、Rᙠp᛻，ᡂj4ᱥ⚞ᨬy⌕ᐹ¢£。]ᦻ¤nẆ¥ḄAFM>ᘤ¦§¨，Nᔠᦋ«ḄAFM┐，STᓣ0ḄAFMᐝ¯§¨，{pT᪶«°ᱯឋ±ᑖ᪆。³´µpT᪶Ḅ☟᝱ᱯឋ，jp¸᝱ᝈºẠ。n⌕Ẇᑁ¼ᒹ¾：✌ᐜ，]ᦻÁÂÃÄḄAFM┐。ÁÂᐙᑖὃ⇋pÈ⚪、ÊÈ⚪、⚣᳛Í᝱È⚪，ÎÏ┐ᐹᨵᔠᳮḄN᪀±Ñ᝱。³ÁÂÎÏAFM>ᘤ¦§¨Rᙠ{⌼ᡂᨬÓÔÕḄÖ×，ØÙ{᪶«°Úi。ᐸ~，jÛÏÜÝḄ´Þᦪà，×´ÞḄáâឋ，{AFM>ᘤ¦

6、§¨«°ᓣ0ᦋ⌼，ÁÂ、ãäåæçâ-è⯧¨，ᦋ«ᡠᵨéêÚiëì±íîï。ᦋ«ð§¨áSØÙ¯ñ3hḄ┐MḄòᔣMôᔣõᐵ§。ᨬð，]ᦻᑭᵨᦋ«ð§¨{T᪶«°。Îᵨ÷ø┐ᙠZøù，{«°úû´Þ，Ïᑮ-ýᦪà，å«°ᑖ᪆ᜐᳮ，Ïᑮᙠ÷ø┐Ḅ☟᝱ᱯឋNÿ。ᡠẆᑴḄÃÄAFM┐Tç⧨RÜدᨵᦔ«°pᱯឋḄ，åᓫ£â，jbâø¯，{4ᱥḄẆᐹᨵ¸iᵨ，{ᑁḄẆ×£ÃḄ⌶。ᐵ：᪶；；ᩰÍ；☟

7、ᱯឋ；-I-ᜧ3ắᦻAbstractThemechanicalpropertiesofbiologicalcellsarerelatedtothestructure,functionandthe“birthanddeath”ofthecellclosely.Thecelliscomposedofmanyorganelles,inwhichthenucleusisthemaincarrierofthegeneticmaterials,andthenucleusplaysavitalroleinthewholec

8、elllifecycle.Thestudyofnuclearmechanicalpropertiescanprovideinsightsintonuclearmythicallife.Whileseveralmethodshavebeendevelopedtocharacterizenuclearmechanicalproperties,aAFM(atomicforcemicroscopy)needlepenetrationtechniqueisdemonstratedtomechanicallycharacterizecellnucle

9、iinsituwithmuchhigheraccuracyandusedmorewidely,andbecomesoneofthemostimportantmethodsofexploring