同种异体嗅球组织移植对神经病理性疼痛大鼠脊髓中p物质表达的影响

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1、摘要舢舢Ⅲ㈣舢㈣删舢Y2427625目的：观察同种异体嗅球组织移植对神经病理性疼痛大鼠脊髓中P物质的mRNA和蛋白水平的变化，探讨P物质在同种异体嗅球组织移植对神经病理性疼痛中的作用。方法：我们将SD大鼠随机分成假手术组(Sham组)、慢性坐骨神经结扎损伤组(CCI组)和CCI+同种异体嗅球组织移植组(CCI+AOBTT组)，于术前和术后1、3、7、14d测定机械缩足反射阈值(MWT)和热缩足反射潜伏期(TWL)，术后7、14d取大鼠术侧腰4(L4)、腰5(L5)脊髓节段，采用逆转录聚合酶链反应(RT．PCR)和免疫组织化学方法检测同种异体嗅球组织移植后CC

2、I组脊髓中P物质(SubstanceP，SP)的mRNA和蛋白水平的变化。结果：CCI组术后7、14天MWT、TwL与Sham组相比明显降低(p<0．05)，脊髓中P物质的mRNA和蛋白水平明显增多(p

3、ive：ToinvestigatethechangeofallogeneicolfactorybulbtissuetransplantationonthelevelofsubstancePmRNAandproteininspinalcordofratswithneuropathicpain，weexploresubstancePinallogeneicolfactoryensheathingtissuetransplantationofneuropathicpain．Methods：WedividedrandomlySDratsintothreegroups

4、：Shamoperation(Sham)group，chronicconstrictioninjury(cci)group，CCI+allogeneicolfactorybulbtissuetransplantation(CCI+AOBTT)group．Mechanicalwithdrawalthreshold(MWT)andthermalwithdrawallateny(TWL)ofratsweremeasuredbeforeoperationandl、3、7and14daysafteroperation．RT—PCR(ReverseTranscripti

5、on—PolymeraseChainReaction)andimmunohistochemistrywereusedtodetectthechangesubstancePmRNAandproteinlevelinL4andL5ofspinalcordofratsontheoperativesidewere7and14daysafteroperation．Results：Comparedwiththeshamgroup，in3，7and14daysMWTandTWLdecreasedmarkedly(p

6、4dayssubstancePmRNAandproteininspinalcordofratsonoperativesideincreasedsignificantlyafteroperationintheCCIgroup(p<0．05)．ComparedwiththeCCIgroup，in3，7and14daysMWTandTWLincreasedmarkedly(p<0．05)inCCI+AOBTTgroup，whilein7and14dayssubstancePmRNAandproteininspinalcordofratsonoperativesid

7、edecreasedsignificantlyinCCI+AOBTTgroup(p<0．05)．Conclusion：AllogeneicolfactorybulbtissuetransplantationgroupmayreduceneupopathicpainofratswithCCIviainhibitiontheexpressionofsubstancePinspinalcord．．Keywords：substanceP：neuropathicpain；RT-PCR；immunohistochemistry；olfactoryensheathingt

8、issue；Rat目录目录第1章引言⋯⋯⋯⋯⋯⋯⋯⋯