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ID:34482598
大小:395.36 KB
页数:7页
时间:2019-03-06
《dna分子标记技术在黄颡鱼遗传学研究中的应用》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、JournalofNanchangInstituteofTechnologyISSN1674—-007614/14PP72-78Voloume30,Number1,February2011ApplicationofDNAmolecularmarkersingeneticresearchofPt'elteobagrusMAOHuiling,XIAOHui,GEXinqi,OUYANGLian(SchoolofLifeScienceandFoodSafety,NanchangUniversity,Nanchang330031,China)Abstr
2、act:TheapplicationofDNAmolecularmarkersingeneticresearchofPelteobagruswasintroducedinthepaper.Itin—volvesgermplasmidentification,phylogeneticevolution,andgeneticdiversity.Furthermore,theexistingproblemsandprospectofDNAmolecularmarkerapplicationwasanalyzedindetail.Keywords:DN
3、Amolecularmarker;Pelteobagrus;geneticdiversity1Background1.1GeneralintroductionofPelteobagrusGenusPeheobagrusbelongstoOsteichtliyes,Siluriofmres,Bagrida[Ij.Asakindofsmallbutfamouseconomicfish,Peheobagrusisgentleomnivoresandhasaremarkableabilitytoadapttoecologicalenvironment.
4、Piswellacceptedbyconsumersbecauseofitstendermeat,richnutrition;highrateoffleshandconvenientcooking:Thus,PeheobagrushasbecomeanimportanteconomicfishspeciesinChina.1.2IntroductiononmolecularnlarkersMostoftheearlyresearchonPeheobagrusfocusedonartificialculturetechnologyanddisea
5、secontrol,fromsuchperspectivesasbiology,physiology,biochemistry,etc.WiththedevelopmentoftheDNAmolecularmarker,thistechnologypropelledthestudiesonPeheobagrus.MoreandmoreresearchersbegantostudyPel~obagrusbyusingDNAmolecularmarkers.DNAmolecularmarkerisakindofgeneticmarkersbased
6、ontheDNApolymorphism.Itcaninheritstablywithsimpleinheritancemodeandreflectsbiologicalcharacteristicsofindividualandpopulation.ComparedwithMorpho—logicalMarkers,CytologicalMarkersandBiochemicalMarkers,DNAmolecularmarkeriswidelyusedsinceitap—peared.Itsadventageslieinitsmultipl
7、etaggedsites,highspecificityandreproducibility,stablegeneticmarker.Thosegreatamountsofinformationwouldnotbeinfluencedbyorganism’sage,developmentalstage,genderorfeed—ingenvironments.DNAmolecularmarkerwouldbecomemoreandmoreimportantinstudyonaquaticanimal,faeili-tatedbytherapid
8、developmentofPCRandelectrophoresistechnology.Soitiscurrentlywidelyappliedin
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