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1、作物学报ACTAAGRONOMICASINICA2010,36(8):1265−1269http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2010.01265小麦品系天95HF2抗叶锈基因定位**周悦李在峰李星王龙张晔刘大群河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心,河北保定071001摘要:苗期基因推导表明,小麦品系天95HF2高抗我国目前
2、多数叶锈菌生理小种。为了确定这一品系所携带的抗病基因,以天95HF2和感病小麦品种郑州5389杂交,获得F1和F2代群体,用叶锈菌小种FHTT和PHTS分别对双亲及其杂交后代进行叶锈抗性鉴定并进行分子标记分析。结果表明,用叶锈菌小种FHTT接种F2代群体时呈现1对显性基因的抗感分离比例,经过亲本和抗感池间标记筛选以及F2代群体的标记检测,Lr1的STS标记WR003和位于5DL的SSR标记wmc443与该抗病基因连锁,遗传距离分别为2.9cM和3.1cM,根据抗性特点和染色体位置推断该基因可能为Lr1。用叶锈菌小种PHTS接种F2代
3、群体时呈现2对基因的抗感分离,分子标记分析结果表明,其中一个基因为Lr1,另一个基因可能为LrZH84。关键词:SSR标记;小麦抗叶锈基因;天95HF2;Lr1;LrZH84MolecularMappingofLeafRustResistanceGenesinWheatLineTian95HF2**ZHOUYue,LIZai-Feng,LIXing,WANGLong,ZHANGYe,andLIUDa-QunCollegeofPlantProtection,AgriculturalUniversityofHebei/Biologica
4、lControlCenterforPlantDiseasesandPlantPestsofHebeiProvince,Bao-ding071001,ChinaAbstract:Thewheat(TriticumaestivumL.)lineTian95HF2appearsinlowinfectiontomostofChinesecurrentpathotypesofPucciniatriticinaatseedlingstage.ForpostulatingtheresistancegenesinTian95HF2,F1andF2p
5、opulationsfromthecrossbe-tweenTian95HF2andZhengzhou5389(susceptibletoleafrust)wereinoculatedwithpathotypesFHTTandPHTSingreenhouse.Theinfectiontypeswereinvestigated15dafterinoculationandmolecularmarkerswerealsousedformappingtheresistancegenes.Wheninoculatingwithpathotyp
6、eFHTT,onlyoneresistancegenewasdetected.Afterscreening1274primerpairsintheparentsandtheresistantandsusceptiblebulks,theresistancegenewasfoundbetweentheintervalofasequencetagsiteforLr1(WR003)andamicrosatellite(wmc443)on5DL.Thegeneticdistancestothetwomarkerswere2.9cMand3.
7、1cM,respectively.ThisresistancegenewasfinallypostulatedtobeLr1.ThesegregationofinfectiontypeinresponsetopathotypePHTSshowedthatthereweretworesistancegenesinTian95HF2.OnewasinferredasLr1,andtheotherwaslikelytobeLrZH84,whichislocatedonchromosome1BL.Keywords:SSRmarker;Res
8、istancegenetowheatleafrust;Tian95HF2;Lr1;LrZH84[4]由小麦叶锈菌(Pucciniatriticina)引起的小麦叶目前已有66个抗叶锈病基因被正式命名,其锈病是影响小麦生产的重要病害之一
