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ID:34466611
大小:287.98 KB
页数:7页
时间:2019-03-06
《DB61∕T 414-2007 饮料中L一苹果酸的测定-酶法》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
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3、<$:~m,~ffij,.~*~:<$:nm~~~~I¥J~~~~Ji:~WJ{Iemis:®Xi~:I¥Jl{tiNfY&:<$:0JLJl!:7f;liBJtJjEr-JCJImX1'~,:!tlfU~T~:<$:J?imT:<$:f5F1{toEN1138-19957J4、tt15、.=======......~oxaloacetate+NADH+H+]~~&~I¥J~OO~~L1jtmlWilioM&~*~if'~~~mz.~~~~oo~~~~.Z9J.~~.M~mz.~!b'$ti.IHGOT)11jHt,~m;z.mi{£L~~U1JZ#{±l¥Jttll)5t,r'$NtJ'.!L:*q:.1ZUH2)aNADH,ffdltWJ!J-;tj'JJLi!'P:m£,e.j;jt,:fW:Fni'±340nmi&K~fl:rr»WittNADH,e.jtaIGOT!i(2)6、Oxaloacetate+L-glutama.::;;;:x:=======......~L-aspartate+2-oxoglutarate5i.itj'f~5,1m.tfiil.Jltti/lP~5.2Li;titM5,3NADDB61rr414-20075.4~It.:e:.Z..*lii.5.5L-MDH5.6HCl5.7NaOH5.8KOH5.9tii*5.1OteiiIitIf)iii8f.lit1lJj*6it.6.1Uji~(5mL1mL50-200~L10-50~L)6.2~t1!7、.no.::)'tt£LOcm6.3~9~~:)'t:)'tJllt,iiIs£jtiE:,f£340nm~IJ:iE6.4.mAi.jm,:fLf£10~6.5il,l.'tIL6.6il'u'i,m¥F,-3.Mi,~ii~lr,.fiIj!k,.Ja.~7~~ljiIT.7.1iitif1jlr,*IJ7.1.1~tfll&:tlilfliiffl50mLm:1A7JC}tff4.75g~i=t~Jtt;fU0.88gL-~.Il,fflNaOHiJj~pHlO.O,fflm:~)tcIEW~8、60mLo7.1.2NADIf1:Hl1fffla1,fItImLm:~7JC}tfdlf35mgT~fiP~NADIt'f.~o7.1.3~1l.-:e:.Z..~~.!H'¥Hlitffla1,ffl0.4mL.m1A7JC~fdlf160U(fJ~.Il-1i£m~Il~T*5}IlP~~.Il-1itm;~Ii~fa:IjIt'f.~o7.1.4L-MDH;~ii.600U/100~L7.1.5HCl:2mollL7.1.6NaOH:2molIL7.1.7KO
4、tt15、.=======......~oxaloacetate+NADH+H+]~~&~I¥J~OO~~L1jtmlWilioM&~*~if'~~~mz.~~~~oo~~~~.Z9J.~~.M~mz.~!b'$ti.IHGOT)11jHt,~m;z.mi{£L~~U1JZ#{±l¥Jttll)5t,r'$NtJ'.!L:*q:.1ZUH2)aNADH,ffdltWJ!J-;tj'JJLi!'P:m£,e.j;jt,:fW:Fni'±340nmi&K~fl:rr»WittNADH,e.jtaIGOT!i(2)6、Oxaloacetate+L-glutama.::;;;:x:=======......~L-aspartate+2-oxoglutarate5i.itj'f~5,1m.tfiil.Jltti/lP~5.2Li;titM5,3NADDB61rr414-20075.4~It.:e:.Z..*lii.5.5L-MDH5.6HCl5.7NaOH5.8KOH5.9tii*5.1OteiiIitIf)iii8f.lit1lJj*6it.6.1Uji~(5mL1mL50-200~L10-50~L)6.2~t1!7、.no.::)'tt£LOcm6.3~9~~:)'t:)'tJllt,iiIs£jtiE:,f£340nm~IJ:iE6.4.mAi.jm,:fLf£10~6.5il,l.'tIL6.6il'u'i,m¥F,-3.Mi,~ii~lr,.fiIj!k,.Ja.~7~~ljiIT.7.1iitif1jlr,*IJ7.1.1~tfll&:tlilfliiffl50mLm:1A7JC}tff4.75g~i=t~Jtt;fU0.88gL-~.Il,fflNaOHiJj~pHlO.O,fflm:~)tcIEW~8、60mLo7.1.2NADIf1:Hl1fffla1,fItImLm:~7JC}tfdlf35mgT~fiP~NADIt'f.~o7.1.3~1l.-:e:.Z..~~.!H'¥Hlitffla1,ffl0.4mL.m1A7JC~fdlf160U(fJ~.Il-1i£m~Il~T*5}IlP~~.Il-1itm;~Ii~fa:IjIt'f.~o7.1.4L-MDH;~ii.600U/100~L7.1.5HCl:2mollL7.1.6NaOH:2molIL7.1.7KO
5、.=======......~oxaloacetate+NADH+H+]~~&~I¥J~OO~~L1jtmlWilioM&~*~if'~~~mz.~~~~oo~~~~.Z9J.~~.M~mz.~!b'$ti.IHGOT)11jHt,~m;z.mi{£L~~U1JZ#{±l¥Jttll)5t,r'$NtJ'.!L:*q:.1ZUH2)aNADH,ffdltWJ!J-;tj'JJLi!'P:m£,e.j;jt,:fW:Fni'±340nmi&K~fl:rr»WittNADH,e.jtaIGOT!i(2)
6、Oxaloacetate+L-glutama.::;;;:x:=======......~L-aspartate+2-oxoglutarate5i.itj'f~5,1m.tfiil.Jltti/lP~5.2Li;titM5,3NADDB61rr414-20075.4~It.:e:.Z..*lii.5.5L-MDH5.6HCl5.7NaOH5.8KOH5.9tii*5.1OteiiIitIf)iii8f.lit1lJj*6it.6.1Uji~(5mL1mL50-200~L10-50~L)6.2~t1!
7、.no.::)'tt£LOcm6.3~9~~:)'t:)'tJllt,iiIs£jtiE:,f£340nm~IJ:iE6.4.mAi.jm,:fLf£10~6.5il,l.'tIL6.6il'u'i,m¥F,-3.Mi,~ii~lr,.fiIj!k,.Ja.~7~~ljiIT.7.1iitif1jlr,*IJ7.1.1~tfll&:tlilfliiffl50mLm:1A7JC}tff4.75g~i=t~Jtt;fU0.88gL-~.Il,fflNaOHiJj~pHlO.O,fflm:~)tcIEW~
8、60mLo7.1.2NADIf1:Hl1fffla1,fItImLm:~7JC}tfdlf35mgT~fiP~NADIt'f.~o7.1.3~1l.-:e:.Z..~~.!H'¥Hlitffla1,ffl0.4mL.m1A7JC~fdlf160U(fJ~.Il-1i£m~Il~T*5}IlP~~.Il-1itm;~Ii~fa:IjIt'f.~o7.1.4L-MDH;~ii.600U/100~L7.1.5HCl:2mollL7.1.6NaOH:2molIL7.1.7KO
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