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1、畜牧兽医学报2008,39(6):6962700ActaVeterinariaetZootechnicaSinica牛ACAA1和ACAA2基因多态性及其与生产性状的关联分析3李恒德,王淑辉,许尚忠,高雪,任红艳,陈金宝(中国农业科学院北京畜牧兽医研究所,北京100193)摘要:以鲁西牛、秦川牛、安格斯、海福特和西门塔尔与蒙古牛杂交牛5个品种共178头为试验材料,通过等位基因特异性聚合酶链式反应(AS2PCR)技术,发现牛乙酰辅酶A酰基转移酶1(ACAA1)基因的A1066G(GenBank登录号:EF576938)和A1452G(Ge
2、nBank登录号:EF576938)2个单核苷酸多态(SNP),其中A1066G突变位于外显子9,是同义突变,A1452G突变位于3′非翻译区(UTR);发现牛乙酰辅酶A酰基转移酶2(ACAA2)基因的C33119T(GenBank登录号:EF583005)单核苷酸多态,位于内含子8上。对ACAA1基因的2个SNP进行分析没有发现单倍型。2个基因多态性与牛生长性状的关联分析表明:ACAA1基因A1066G突变位点的3种基因型间眼肌面积差异显著(P<0105),AA型最高,AG型次之,GG型最低;ACAA1基因A1452G突变位点的3种基
3、因型间眼肌面积差异显著(P<0105),GG型最高,AG型次之,AA型最低;ACAA2基因C33119T突变3种基因型间日增重差异极显著(P<0101),其中CT型最高,TT型次之,CC型最低;眼肌面积差异显著(P<0105),其中CC型最高,TT型次之,CT型最低。关键词:ACAA1;ACAA2;牛;AS2PCR;生产性状中图分类号:S82312文献标识码:A文章编号:036626964(2008)0620696205AssociationAnalysisofBovineACAA1andACAA2GenePolymorphismswi
4、thProductionTraits3LIHeng2de,WANGShu2hui,XUShang2zhong,GAOXue,RENHong2yan,CHENJin2bao(InstituteofAnimalScience,ChineseAcademyofAgriculturalSciences,Beijing100193,China)Abstract:178cattlefromfivebreeds,Luxi,Qinchuan,Angus,HerefordandcrossbreedofSim2mentalandMenggolianwere
5、usedintheexperiment.Throughallele2specificpolymerasechainre2action(AS2PCR),twosinglenucleotidepolymorphisms(SNP):A1066G(GenBankAccessionNo.EF576938)andA1452GwerefoundonACAA1gene.A1066GSNPislocatedatexon9,butitisasilentmutation,whileA1452GSNPislocatedat3′2untranslatedregi
6、on(UTR).C33119TSNP(GenBankAccessionNo.EF583005)wasfoundatintron8ofACAA2gene.NohaplotypewasfoundbetweenthesetwoSNPsofACAA1gene.ResultsofassociationanalysisoftheseSNPswithproductiontraitsshowedthatA1066GsubstitutionofACAA1isassociatedsignificant2ly(P<0105)withloin2eyearea,
7、themeanvalueofgenotypeAAisthehighest,genotypeAGsecondhighestandgenotypeGGthelowest.A1452Gsubstitutionisassociatedsignificantly(P<0105)withloin2eyearea,themeanofgenotypeGGisthehighest,genotypeAGsecondhighestandgenotypeAAthelowest.TheC33119Tsubstitutionisassociatedsignific
8、antlywithdailygain(P<0101)andloin2eyearea(P<0105),fordailygain,themeanofgenotypeCTisthehighest,收稿日期:200
