生物光学在蛋白质组学中的应用

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1、CHAPTER15BIOPHOTONICSAPPLIEDTOPROTEOMICSMICHELFAUPEL,DÉBORABONENFANT,PATRICKSCHINDLER,ERICBERTRAND,DIETERMUELLER,MARKUSSTOECKLI,FRANCISBITSCH,TATIANAROHNER,DIETERSTAAB,andJANVANOOSTRUMNovartisInstitutesforBiomedicalResearch,BaselTableofContentsAbstract............................................

2、.................................3231.Introduction.....................................................................3232.Two-DimensionalElectrophoresis.............................................3243.ProteomicsAnalysisbyMassSectrometry....................................3254.MassSpectrometry

3、Imaging....................................................3294.1.PrinciplesandMethods...................................................3304.2.CompoundImaging.......................................................3314.3.PeptideImaging...........................................................33

4、24.4.ProteinImaging...........................................................3335.Conclusions.....................................................................336References..........................................................................337Abstract:Sincethecompletionofthehumangenome

5、sequencing,ourunderstandingofgeneandproteinfunctionandtheirinvolvementinphysiopathologicalstateshasincreaseddramati-cally,partlyduetotechnologicaldevelopmentsinphotonics.Photonicsisaveryactiveareawherenewdevelopmentsoccuronaweeklybasis,whileestablishedtoolsareadaptedtofulfilltheneedsofotherdisci

6、plineslikegenomicsandproteomics.Biophotonicsemergedattheinterfaceofphotonicsandbiologyasaverystraightforwardandefficientapproachtoobserveandmanipulatelivingsystems.Inthischapter,wereviewthecurrentapplicationsofphotonicsandimagingtoproteomicsfrom2Dgelsanalysistomolecularimaging.1.INTRODUCTIONThec

7、ompletesetofgenesofanorganismcomposesitsgenome,whilethecompletesetofproteinsofacellortissueconstitutesitsproteome.Whereasthegenomeofcellsremainsstablethroughtheirlifetime,thecellularproteomechangesinresponsetothe323E.Bertran