病原侵染早期小麦抗白粉病性状的构成因素剖析和qtl定位分析

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1、作物学报ACTAAGRONOMICASINICA2011,37(7):12191228http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2011.01219病原侵染早期小麦抗白粉病性状的构成因素剖析和QTL定位分析王华忠章珍贺洋岳洁瑜天津师范大学生命科学学院/细胞遗传与分子调控天津市重点实验室,天津300387摘要:以国际小麦作图组织提供的W7984×Opata85重组近交群体为材料,将白粉病抗性分解为互作早期不

2、同时间点的乳突指数、乳突级别、吸器指数和二级菌丝指数等成分性状,在成分性状鉴定和统计的基础上,进行遗传分析和相关QTL定位。白粉菌侵染早期乳突指数和吸器指数随时间的变化趋势均受主效单基因的调控。数量性状分析共找到34个与抗白粉病相关的QTL(21个主效QTL),分布于小麦1B、1D、2B、3A、3B、3D、4A、4B、4D、5B、6A、6B、6D、7B和7D染色体上。位于7B染色体上的QTL(QPmPI16.tn-7B)对乳突形成的影响极为显著,贡献率达48.7%,促进乳突形成的等位变异来自Opata85。不同成分性状存在共定位的QTL。成分性状的特异QTL提供了更多有关抗白粉病

3、遗传机制的信息。关键词:小麦白粉病;侵染早期;成分性状;QTL定位DissectionandQTLMappingofComponentTraitsofResistancetoWheatPowderyMildewatEarlyInfectionStageWANGHua-Zhong,ZHANGZhen,HEYang,andYUEJie-YuSchoolofLifeSciences/TianjinKeyLaboratoryofCyto-genetical&MolecularRegulation,TianjinNormalUniversity,Tianjin300387,ChinaAbs

4、tract:PowderymildewcausedbyBlumeriagraminisf.sp.tritici(Bgt)isoneofthemajorfungaldiseasesofwheat,andtheuseofresistantcultivarsisthemosteffectivediseasecontrolapproach.Here,theInternationalTriticeaeMappingInitiative(ITMI)W7984×Opata85wheatrecombinantinbredpopulationwasusedforpowderymildewresis

5、tancegeneticstudy.Resistancecom-plextraitwasdissectedintocomponenttraitsaspapillaindex(PI),papillascale(PC),haustoriumindex(HI)andsecondaryhyphaindex(SI)ofseveralsuccessivetimepointatearlystageofBgtinfection.ThechangetrendsofPIandHIwithtimewerebothcon-trolledbysinglemaineffectgenes.Withquanti

6、tativetraitanalysis,34resistance-relatedQTLsincluding21maineffectQTLswerefoundanddistributedinwheat1B,1D,2B,3A,3B,3D,4A,4B,4D,5B,6A,6B,6D,7B,and7Dchromosomes.QTLQPmPI16.tn-7B,whichregulatedtheformationofpapilla,hadthelargesteffect,singlyaccountingfor48.69%ofthephenotypicvariance.LocusofQPmPI1

7、6.tn-7BpromotingpapillaformationwasderivedfromtheparentOpata85.ColocalizedQTLswerefoundamongdifferentcomponenttraits.Componenttrait-specificQTLsmayprovidemoreinformationaboutthegeneticmecha-nismofwheatpowderymildewresistance.Keywords:Wheatpow