4.4 nonlinear optical imagingnew

《4.4 nonlinear optical imagingnew》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、BrittonChanceCenterforBiomedicalPhotonicsFundamentalsofbiophotonicsChapter4Bioimaging:PrinciplesandTechniquesInstructor:LingFuCollegeofOptoelectronicScienceandEngineeringWuhanNationalLaboratoryforOptoelectronicsBrittonChanceCenterforBiomedicalPhotonicslfu

2、@mail.hust.edu.cn1BrittonChanceCenterforBiomedicalPhotonics4.4Nonlinearopticalmicroscopy(非线性光学显微成像)成纤维小鼠小细胞脑组织细胞分小鼠内裂间期耳毛细胞2BrittonChanceCenterforBiomedicalPhotonicsMechanismofNonlinearopticalprocesses(1)(2)2(3)3PEEEPPolarization(极化率)ofthecorresp

3、ondingmediumEStrengthoftheelectricfield(i)ithordernonlinearsusceptibility(非线性极化系数)Linearopticaleffects（线性光学效应）(1)PEAbsorptionandreflectionNonlineareffects（非线性效应）(1)(2)2(3)3PEEEharmonicgeneration（谐波）,fourwavemixing（四波混频）,crossphasemodulation（交叉

4、相位调制）,multiphotonabsorption（多光子吸收）,parametricamplification,stimulatedRamanscattering（受激拉曼散射）……3BrittonChanceCenterforBiomedicalPhotonicsFeaturesofnonlinearopticalmicroscopy4BrittonChanceCenterforBiomedicalPhotonicsNonlinearopticalmicroscopyMultiphotonexc

5、itationfluorescencemicroscopy（多光子激发荧光显微成像）Second-harmonicgenerationmicroscopy（二次谐波显微成像）Coherentanti-stokesRamanscatteringmicroscopy（相干反斯托克斯拉曼散射显微成像）5BrittonChanceCenterforBiomedicalPhotonicsNonlineareffect:Multiphotonabsorptionλ(μm)hv(eV)2006.02505.0300

6、4.0SinglePhoton4003.0EmissionTwo-photonabsorptionprocessTwoPhoton6002.0800ThreePhoton1.0χ(5)medium3P2P1PExcitationWavelengthbandThree-photonabsorptionprocess6BrittonChanceCenterforBiomedicalPhotonicsAdvantagetheofmultiphotonmicroscopy(1)Near-infraredligh

7、tsourceTi:SapphireLaser（ultrashortpulsedlaseratfemtosecondtimescale）DeeptissueimagingLessphotodamage800nm7BrittonChanceCenterforBiomedicalPhotonicsAdvantagetheofmultiphotonmicroscopy(2)Inherentopticalsectioningability8BrittonChanceCenterforBiomedicalPho

8、tonicsAdvantagetheofmultiphotonmicroscopy(2)Localizationofexcitation(a)(b)ObjectObjectlenslensFocus(c)FocusPlaneSamplePlaneSampleSinglephotonexcitationTwophotonexcitationSinglephotonversustwophotonexcitationvolume9B