Calcium flash-ROS-cell-2008-Cheng heping.pdf

《Calcium flash-ROS-cell-2008-Cheng heping.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、SuperoxideFlashesinSingleMitochondriaWangWang,1,*HuaqiangFang,4LindaGroom,5AiwuCheng,2WanruiZhang,4JieLiu,4XianhuaWang,4KaitaoLi,4PeidongHan,4MingZheng,4JinhuYin,3WeidongWang,3MarkP.Mattson,2JosephP.Y.Kao,6EdwardG.Lakatta,1Shey-ShingSheu,5KunfuOuyang,7JuChen,7RobertT.Dirks

2、en,5andHepingCheng4,*1LaboratoriesofCardiovascularSciences2Neurosciences3GeneticsNationalInstituteonAging,NationalInstitutesofHealth,Baltimore,MD21224,USA4InstituteofMolecularMedicineandNationalLaboratoryofBiomembraneandMembraneBiotechnology,PekingUniversity,Beijing100871,

3、China5DepartmentofPharmacologyandPhysiology,UniversityofRochester,SchoolofMedicineandDentistry,Rochester,NY14642,USA6DepartmentofPhysiology,UniversityofMarylandSchoolofMedicine,Baltimore,MD21201,USA7DepartmentofMedicine,UniversityofCalifornia,SanDiego,CA92093,USA*Correspon

4、dence:chengp@pku.edu.cn(H.C.),wangwang@umich.edu(W.W.)DOI10.1016/j.cell.2008.06.017SUMMARY1998).However,increasingevidenceindicatesthathomeostaticandphysiologicallevelsofROSareindispensableinregulatingInquiescentcells,mitochondriaaretheprimarydiversecellularprocessesinclud

5、ingionchannel/transporter2+sourceofreactiveoxygenspecies(ROS),whicharefunction(ZimaandBlatter,2006),Casparkproduction(Isaevageneratedbyleakinessoftheelectrontransportetal.,2005;Yanetal.,2008),proteinkinase/phosphataseactiva-chain(ETC).HighlevelsofROScantriggercelldeath,tio

6、n,andgeneexpression(Droege,2002).Theemergingviewiswhereaslowerlevelsdrivediverseandimportantcel-thatROScontributetomultipleessentialintracellularsignalingprocessesrangingfromcellmetabolismtoischemicprecondi-lularfunctions.Weshowherebyemployinganewlytioning(Droege,2002;Otan

7、i,2004).developedmitochondrialmatrix-targetedsuperox-Inquiescentcells,ROSareprimarilyproducedasbyproductsideindicator,thatindividualmitochondriaunder-ofmitochondrialrespirationwhenelectronsleakfromtheelec-gospontaneousburstsofsuperoxidegeneration,trontransferchain(ETC)(Wal

8、lace,2001a,b).Superoxideaniontermedsuperoxideflashes.Superoxideflashes(O)istheprimaryROSg