大鼠内嗅皮层微注射orexin-a对睡眠—觉醒及空间记忆的影响

《大鼠内嗅皮层微注射orexin-a对睡眠—觉醒及空间记忆的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、—————————————————皇至兰型至塑±堂垡笙茎OX2Rorexinreceptor2orexin受体2SCRrAAV2／1一SCR．shRNA—EGFPOR1rAAV2／1一OR1一shRNA．EGFPT-mazeT迷宫Open—field旷场mMmilimole毫摩尔mlmililiter毫升minminute分ggram克dday天ssecond秒'笙三至堕奎堂堡±堂笪笙壅一TheeffectofOrexinAinentorhinalcortexonsleep—wakecycleandspatialmemoryAbstract0rexin．alsoknow

2、nashypocretin，isakindofneuropeptideproducedbyorexmneuronsinthelateralhypothalamus．Thetwomonomersorexin—Aandorexin-BareDrocessedfromasinglepre．pro．orexinpolypeptidesynthesizedfromonesinglegene·Orexin—Aconsistsof3aminoacidsvdthtwodisulfidebonds．Bothorexin—IreceptorfOX1R、andorexin一2receptor

3、(OX2R)areG—proteincoupledreceptorsandunderlietheorexin-mediatedphysiologicalroles．TheaffinitybetweenOXlRandorexin‘Aishigherthanthatoforexin-B，whereastheaffinitybetweenOX2Randorexin‘Aisidenticalwiththatoforexin-B．orexinneuronsprojectbroadlythroughoutthewholebrain，whichisofgreatsignificanc

4、einthemaintenanceofwakefulness．Furthermore，thenarcolepsycanbef’oundintheorexinknockout(KO)ororexinreceptortranscription—Disruptedmice·Atthesametime。theprojectionfromorexinneuronstohippocampus(Hp)wasfound，whichsuggestedthatorexinmighthasanimportantroleinthespatiallearningandmemory·Theento

5、rhinalcortex(EC)hasbeenregardedasanodalpointbetweenhippocampusandneocorticalcortex．Generally，superficiallayersofEC(1ayersII—III)deliveriesamultitudeofinputsfromV—VI)transportsoutputsotherbrainregionstohippocampus，andthedeeplayers(1ayersfromhippocampus，mainlytheCA1andsubiculum，totheneocor

6、ticalcortex．Themedialentorhinalcodex(MEC)conveysthespatialinformation，whilethelateralentorhinalcodex(LEC)transfersnon—spatialinformationlikeolfactoryandsomatosensorysignalstohippocampus．TheseanatomicalpropertiesmaketheECindispensableintheneocorticalcortex—hippocamplenetwork—mediatedencod

7、ing，consolidationandretrievalofmemories，especiallythespatialmemory·Thepolysomnographyrecordingandmicro—injectiontechniqueshavebeenappliedinourexDeriments．Firstly，weexploredtheeffectsofexogenousorexinAonratsleep，thenwemana￡edtodeterminetheeffectsoforexinAonthespecialmemory