孕妇血清及胎盘fabp4水平变化与子痫前期

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1、英文摘要group舢and26womenwithmoderatepreeclampsia(MPE；groupB)．25healthypregnantwomenweretakenascontrolgroup(groupC)．Extractionofpreeclampsiagroup(groupAandB，beforedrugtreatment)andcontrolgroupofpregnantwomeneach6mlperipheralvenousblood．Coagulationtube3ml，2000RPMcentri

2、fugal10minutes，draintheupperseruminEPtube，placedina．80℃refrigerator,untilthesamebatchofdeterminationofserum1evelofFABP4．Another3mlincoagulationtubeforthedetectionofTG，(triglycerides，TG)，CHOL(totalcholesterol，CHOL)，HDL(hi曲densitylipoprotein，}玎DL)，LDL(10wdensitylip

3、oprotein，LDL)，GLU(glucose，GLU)etc．Wthin30minutesaterdelivery，placentalfragmentsapproximatelylcm’insizeweresharplydissectedatasitemidwaybetweenthecordinsertionandthelateralplacentalmarginandmidwaybetweenthechorionicandbasalplates．4％formaldehydefixed24hoursforparaf

4、finembeddingforHEstainingandimmunohistochemistry．TheserumlevelofFABP4wasdetectedbyenzyme．1inkedimmunosorbentassay(ELISA)．HDL，LDL，TG，CHOL，biochemicalindexdeterminationbyautomaticbiochemicaldetector．Andrecordthebloodpressure，height，weightandotherindicators．Respecti

5、velyineachgrouptake10subjectsusingimmunohistochemicalstainingmethodtodetectFABP4proteinexpressioninplacentatissues，andappliedtheImage—proplus6．0imageanalysissoftwaretocalculatestheaverageopticaldensityvalueFABP4proteinineachgroup．AllthedatawereanalyzedbySPSS13．0s

6、oft-ware．Results：1TheserumlevelsofFABP4ingroupA，groupBandgroupCare(9．71+3．94)ng／ml，(7．44+3．35)ng／ml，(5．20士3．82)ng／m1．underpairwisecomparison，allhavesignificantdifference衅0．05，火O．001，氏O．05)．FABP4levelofgroupA[(9．71士3．94)ng／ml】issignificantlyhigherthangroupC[(5．24-

7、3．82)ng／m1]，withsignificantstatisticaldifference(P<0．001)．2Amongtheindexoflipidmetabolism：SerumTGlevelsingroupA，groupBandgroupCgraduallydecline，SerumTGlevelinGroupAandgroupB[(4．98士1．75)，(3．944-1．25)mmol／L】issignificantlyhigherthan英文摘要groupC[(2．41土0．83)mmol／L]．Com

8、paredrespectively，Differencesallhavestatisticalmeaning畎0．001，P<0．001)．ComparedgroupAwithgroupB，difl氏。hwstatisticalsignificance(．05)．SerumHI)Llevelsltterenceals