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1、ProteinPurificationHandbook18-1132-29EditionABHiTrap,Sepharose,STREAMLINE,Sephadex,MonoBeads,MonoQ,MonoS,MiniBeads,RESOURCE,SOURCE,Superdex,Superose,HisTrap,HiLoad,HiPrep,INdEX,BPG,BioProcess,FineLINE,MabTrap,MAbAssistant,Multiphor,FPLC,PhastSystemandÄKTAaretrademarksofAmershamPhar
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3、pliesthem.Acopyofthesetermsandconditionsofsaleisavailableonrequest.©AmershamPharmaciaBiotechAB1999-Allrightsreserved.AmershamPharmaciaBiotechABSE-75184UppsalaSwedenAmershamPharmaciaBiotechUKLimitedAmershamPlaceLittleChalfontBuckinghamshireEnglandHP79NAAmershamPharmaciaBiotechInc800
4、CentennialAvenuePOBox1327PiscatawayNJ08855USAProteinPurificationHandbookContentsIntroduction........................................................................................................7Chapter1PurificationStrategies-ASimpleApproach.......................................
5、...............9Preparation............................................................................................10ThreePhasePurificationStrategy..........................................................10GeneralGuidelinesforProteinPurification................................
6、............12Chapter2Preparation......................................................................................................13BeforeYouStart....................................................................................13SampleExtractionandClarification.............
7、.........................................16Chapter3ThreePhasePurificationStrategy....................................................................19Principles................................................................................................19SelectionandCombination
8、ofPurificationTechniques..
