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1、产卵与孵化：研究发现8窝卵，共99枚卵。产卵时间主要集中在10:00~15:00之间，夜间未发现筑巢行为，卵通体白色透明，质地为革质，较软，窝卵数为12.4±1.7枚（n=8，10~15枚），与腹甲长度呈线性相关（P<0.05）；99枚卵成功孵化出11只幼体，其中36枚未受精，龟卵主要被台湾小头蛇（Oligodonformosanus）、印大头蚁（Pheidoleindica）和双齿多刺蚁（Polyrhachisdives）捕食，稚龟颜色鲜艳，土壤粘附于身体上，耳后两条红色斑纹不明显，卵黄尚未完全吸收，活动能力较弱；平均孵化温度27.
2、7℃（27.1℃~28.8℃），平均孵化时间70.3d±9.2d（57d~78d）。综合本次研究的结果可知，红耳龟在海南岛自然条件下建立了可繁育后代的种群，卵泡发育数量多，一个繁殖周期内可发育多次，巢址选择范围广，繁殖能力强，天敌少。首次描述红耳龟在入侵地的产卵行为。红耳龟的这些繁殖特性将对本地淡水龟的生存产生威胁，造成本地生物多样性的减少，相关部门应该尽快制定相关法律，以防治该物种的进一步繁殖扩散。关键词：红耳龟；繁殖；卵泡发育；超声波技术；巢址选择；外来物种；海南岛IIAbstractTheRed-earedturtle(Trac
3、hmysscriptaelegans)wasoneoftheworld’smostdangerous100invasivespecies.Therewastherelativelysystematicandcomprehensiveresearchinthecountryoforigin,includingmorphology,physiologyandreproductivebiologyandsoon.Inchina,theresearchstartedlateandlessofthespecies,includinghabita
4、t,homerang,feedinghabitsinshort-termstudies,buthadyettoseethewildbreedingresearch.Understandingofreproductivebiologycharacteristicsofinvasivespeciesplayedakeyroleforinvasivespeciessuccessfulcolonizationanddiffusion.Thatsizeofthefertilityforinvasionsuccessisofgreatsignif
5、icance.Wecarriedoutafieldbreedingecologicalresearchofalienspecies,T.scriptaelegans,fromOctober2011toSeptember2012,atShazhouIslandofWanquanRiver,Hainan.Mainresearchresultswereasfollows:Folliculardevelopment:thesampleswereobtainedbytrapped,andthesamplesweredetectedthenumb
6、erandsizeoffolliclesbyultrasonictechnique.Theresultsshowedthat54ultrasonicimagesoffollicularofthesamplesweredetected,whichwasdetectedin31individualsonlyonetime,sevenindividualstesttwotimes,threeindividualstestedthreetimes.Theaverageofsizeoffollicleswasgreaterthan9.0mm(6
7、.0mm~33.7mm,n=426)ineachmonth.AndfolliclesgrowthbeganinSeptemberandreachedamaximumsizeinJune(16.6±3.9mm，n=39),withmaximumnumberinMarch(45±4，n=4).Instudyarea,weforecastedthatT.scriptaelegansreproduced2.48clutchesperyearduringbreedingseason.Nestsiteselectionandcharacteris
8、tics:Theprocessofselectinganestingsiteisimportanttofemalesinanimmediatesensebecausetheyaremorevulnerabletoterr
