大鼠缺血性脑水肿早期aqp4mrna表达的动态变化分析

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1、(P<0。05)。实验同时显示脑水含量与AQP4mRNA表达呈正相关(r=O。871，P

2、·eartysta殳eO薹iscnemicbrainedemainratsObjectiveToinvestigatetheexpressionofaquaporin-4(AQP4)mRNAintheearlystageofcerebraledemafollowingischemiainratsandelucidatetherelationshipbetweenthedynamicchangesofbrainwatercontentandexpressionofAQP4mRNA．TheresultsmayrevealthefunctionandeffectofAQP4inearly

3、stageofischemicbrainedemaandbebeneficialtofindanewapproachforthetherapyofcerebraledema．Methodsl。Materials1．1Animalandgrouping：66healthyWistarratswererandomlyassignedtopseudo—operationgroup，operationgroup，TTCstaininggroup．EachgroupWasdividedintosixsub-groupaccordingtodifferenttimepoints(1h，3h，6

4、h,12h，18h，24h)．Everysubgroupcontainedfiverats．1．2Mainagentsandapparatus：RNAPCRKit，PCRamplificationsystem，gelimaginganalysissystem，ultravioletspectrometer．1，3Animalmodel：ArevisedmodelofLinehitchlawaccordingtoZeaLonga．1．4TTCstaining：Itdemonstratesthemodelissucceed．2。Indexdetection2．1Brainwaterco

5、ntent：detenmnedbywet-dryweight．2．2DetectingofAQP4mRNAinbraintissue：11∽expressionofAQP4mRNAwasassessedbyRT—PCR．RNA1uLwasextractedfromthebraintissue，addingrelatedreagents。TheeDNAwassynthesizedbymRNAthroughreversetranscription，mixedtheeDNAwithAQP4specificprimersandrelatedreagents，during40secondsw

6、ith94"C，1minutewith50℃，lminutewith72"C，after35cycles，extension7minutes晰也72℃，theamplifiedproductsofAQP4Wasmixedwithequal13-actinamplifiedproducts，thenbrought5ulmixedproductsin2％agarosegelbed，electrophoresis，the4ratio(GrayOftheratio)ofAQP4mRNAtotheopticaldensityintegralof13-actinelectrophoresisp

7、roductswereobservedandcalculatedbygelimaginganalysissystemobservationandcalculation，whichdeterminedtheexpressionlevelAQP4mRNA．3。StatisticalprocessingAlldatawereanalyzedbySPSSI3．0statisticalsoft．ThedatafromeverygroupwerepresentedbyX+S，th