牙龈卟啉单胞菌rag位点基因分型与慢性牙周炎的相关性分析

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1、>0．05)。rag-1，3型在中重度组的联合检出率高于轻度组(尸O．05)。3、rag-1基因在不同牙周炎症状态-FRNA表达检测结果轻度组、中度组和重度组酏Jrag-1型基因对蛆表达水平分别显示增强，减弱和增强。轻度组和重度组rag-1型基因的RNA表达水平与牙周炎症状态呈正相关，而与中度组则呈负相关。结论1、不同，．昭基因型的尸gingivalis在慢性牙周炎患者龈下菌斑中的分布有所不同。rag-1型的检出率最高，其次为rag-3型，表BJ目rag-1型和，．纷3型基因与我国人群慢性牙周炎的发生发展密切相关。ra

2、g-1型在中重度组的检出率高于轻度组，表明携带rag-1型基因的户gingivalis更能造成牙周组织严重的破坏，与中重度慢性牙周炎密切相关。2、rag-1型基因在轻度组、中度组和重度组的RNA表达水平分别呈现增高．降低一增高的趋势，表BJgrag-1型基因的RNA表达水平随着牙周组织破坏程度及炎症状态的不同而改变。关键词慢性牙周炎；牙龈卟啉单胞菌；rag；基因型3·英文论著摘要·TheCorrelationAnalysisoftheragLocusGenotypesofP,"phjgingivalisandChronicPeriodontitisoIt"orpltyromona

3、sgmgivalisandhroniceriodontitisobjectiveTheaimofthepresentstudieswastodetectthedispositionofPorphyromonasgingivalis(P．gingivalis)offourdifferentraglocusgenotypes，andtodetecttheexpressionofrag-1inRNAlevelindifferentperiodontalinflamedconditionsbypolymerasechainreaction(PCR)andreversetranscript

4、ion—polymerasechainreaction(RT-PCR)．WediscussedthedispositionregularityoftheraglocusgenotypesofPorphyromonasgingivalis，andtheexpressionregularityofrag-1inRNAlevelindifferentperiodontalinflamedconditions．Methods1．StudysubjectsAtotalof50subjectswereenrolledinthestudieswhowereselectedfromtheperi

5、odontaldepartmentofSchoolofStomatology，ChinaMedicalUniversity．Subjectswereexcludedfromthestudiesifanyofthefollowingconditionsapplied：systemicdisease，pregnancy，professionaltoothcleaning，periodontaltherapyduring1yearortakinganymedicineduring3monthsprecedingtothestudy．Allsubjectsenrolledintothes

6、tudiessignedtheinformedconsentform．2．MeasurementofclinicalperiodontalconditionWeselectedthreelesionstatusesinchronicperiodontitissubjects．ClinicalparametersincludedProbingDepth(PD)、clinicalattachmentloss(CAL)andthelossofalveolarbonebyX-ray．Alltheclinicalparametersweremeasuredandrecordedbyasin

7、gleexaminer．43．SamplescollectionSubgingivalplaquesampleswereoNainedwithsteriletoothpicksintolmlofPBSforDNAandRNAisolation．4．SamplesgroupingThesamplesof50subjectsweredivideintotwogroups，whichwerelightgroupandmoderately-severelygroupaccordingto