一例人体寄生包虫以及我国不同地区包虫的鉴定

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1、第二军医大学硕士学位论文sequences，XJ—Eg，YL—Em，422，450，317，133，I，IIandIIIhadasimilarityof97％，92％，93％，93％，92％，92％，92％，93％，and93％、)~ritllEgranulosus，respectively．ForND1sequences，XJ—Eghadasimilarityof99％toEgranulosus，422，450hadasimilarityof99％toEmultilocularis，317，133hadasimilarityof98％toEshiquicus,andI，

2、IIandIIIhadasimilarityof98％toEmultilocularis．A1ltheresultsdemonstratedthatCHEmWaSaunusualparaSitism，orvariantstrainofEgranulosus．Thereasonforthisismightbecauseitparasitizedoutsideofthegastricwallinthesacoflesseromenta,withlessbloodsupply．Theblastingresultsshowedthatthree18SrRNAsequencesam

3、plifiedfromCHEmhadthemostsimilaritytoEgranulosus(Thereisnoother18SrRNAsequencesofEchinococcustapewormsubmittedinGenBanKexceptforEgranulosus)．BasedonblastingonNDlsequences，ourresultsallocatedsamplesfromdifferentareasintofollowing4groups：SamplesfromInnerMongolia(I、II、III)areallhavethepolylo

4、cularhydatid，andinthephylogenetictree，．TheywerelocatedinGroup1，closelyrelatedtoE．multilocularis；Group2isE．multiloculariswhichincludessamplesofYL-Em，450，422；Group3isEshiquicus，thesamplesincludes317，133；Group4isEgranulosus，thesamplesareXJ-EgandCHEm．Inconclusion，bymeansofamplificationofthere

5、lativelyconservative18SrRNAsequencesusingtheuniversalprimersofeukaryote，andtheND1sequencesofmitochondfionusingthespecificprimersoftheEchinococcus，wecandiscriminationEgranulosus，EmultilocularisandEshiquicus．Thepatient’SsampleWaSidentifiedasEgranulosus．Onthewhole，thisinvestigationhadprovide

6、dreliableexperimentaldataforprecisediagnosingofhumanhydatiddiseases嬲wellasusefulmethodforidentificationofparasites．T11isisthefirstsystematicresearchonthephylogeneticanalysisofEchinococcustapeworm幢．granulosus，E．multilocularis，E．shiquicus，E．sibiricensis，E．russicensis，andEmultilocularis)inou

7、rcountry．KEYWORDS：hydatidtapeworm，moleculemarkers，18SrRNA，mtDNAND1，identification．6．一例人体寄生包虫以及我国不同地区包虫的鉴定．土上——L．月IJ舌棘球绦虫在动物分类学上属于绦虫纲(Cestoda)、圆叶目(Cyclophyllidea)、带科(Taeniidae)、棘球属(GenusEchinococcus)。该属现有4个公认的独立种，即细粒棘球绦虫(Echinococcusgranulosus)、多房棘球绦虫(Emultilocularis