山葡萄种质资源抗寒性评价方法的建立及评价的研究

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1、万方数据AbstractBasedonthenationalfruitgermplasmHtisamurensisRupr．nurseryplatform，one—year-oldbranchesof39germplasmresourcesof形amurensis，2矿amuens西-Europeanhybrids(ZuoYou—hong，BeiBing-hong)and1hybrid(Beta)ofAmericanHtisastestmaterials，inthisstudyweevaluatedsystematicallythecold—resistanceof"am

2、urensis，establishedthemethdforevaluationofcold—resistanceof形amurensisanddividedthecold—resistanceof彤amurensisgermplasm．Andwealsoexploredtherelationshipbetweencold—resistanceandcultivationcharacteristicsofgermplasmresourcesof矿amurensis．Themainresultsshowedasfollows：1．Thebestmethodaboutmeas

3、uringRECWasdetermined，itWasthatfirstlybrancheswerepeeled，thenRlWastestedafterstandingextraction3horvacuumpumping40min，orvibratingextraction1h，andR2Wastestedafterstandingextraction8hafterboilingwaterbath。Besides，wefoundthatamongphysiologicalandbiochemicalindexesaboutcoldresistanceofbranch，

4、thecontentofMDAandRECincreasedasthe‘S’typecurveastemperaturedecreased，therestindexeschangedassinglepeakcurveastemperaturedecreased．AndthereweresignificantpositivecorrelationsbetweenthecontentofMDAandREC，thecontentofprolineandsolublesugar,thecontentofprolineandsolubleprotein，thecontentofso

5、lublesugarandsolubleprotein．However,allindexesweresignificantlycorrelatedwithitsaveragesubdinativelevel．TheaveragesubdinativelevelfromsubordinatefunctioncouldbethecomprehensiveindexforthecomprehensiveevaluationofcoldresistanceofgermplasmresourcesofKamurensis．Underlowtemperaturetreatments．

6、theCurvesofaveragesubdinativelevelcouldbetteridentifythecoldresistanceofdifferetgrapevarieties．2．Accordingtoaveragesubdinativelevel，thereweredifferencesincoldresistanceamongtestedgermplasmresourcesofⅣamurensis：73099belongedtothehighresistancegermplasm，anditsaveragesubdinativelevelWas0．71；

7、Therewere1germplasmsbelongingresistancegermplasm，whoseaveragesubdinativelevelwereO．50～0．67．Therewere23germplasmsbelongingmiddleresistancegermplasmandonly3germplasmsbelonginglowerresistancegermplasm．However,comparedwith形amurensis．the2Kamuens括-Europeanhybridsand1hybri