促红细胞生成素在脑缺血再灌注损伤中对神经元线粒体的影响与作用机制的分析

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1、Chapter3．ProtectiveEffectsofErythropoietinonMitochondrialFunctionandStructureofSDRatsAIMS：ToobserveErythropoietin’SprotectiveeffectsonmitochondrialfuctionandstructureofSDratsduringbrainischemia／reperfusioninjury．METHODS：FortySDratsarerandomlydividedintofourgroups：EPOgroup(n。lO)，lschemia／reperfusion

2、group(n=l0)，Controlgroup(n21o)andShamgroup(n。lO)．Modelsofleftcerebralischemia—reperfusion(2hours)wereduplicatedInEPOgroupandischemia／reperfusiongroup．EPOgroupweretreatedwithintraperitonalinjectionofEPOatadoseof3000U／Kgmixedwiththesamedoseofsaline，whiletheIschemia／reperfusiongroupwiththesamedoseofsa

3、line．TheratsinShamgroupwereonlytreatedbycarotidarterialisolationwithoutischemiareperfusionorintraperitonealinjectionofanything．AndnothingwasdonetoControlgroupbeforeRatsarekilled．ThechangesactivityofNa+一K+-ATPaseintheneuronalmitochondria，andCaspase一3postiveneurons’numberbymeansofimmunohistochemistry

4、，Mitochondrialmembranepotentialandmitochondrialstructurewereobservedat72hourspostoperativelyinallgroups．Result：(1)mitochondrialmembranepotential、Na+一K+-ATPase：ItisobviouslyhigherinEPOgroupthanthatinischemia／reperfusiongroup．(2)NumberofCaspase一3postiveneurons’：ItisobviouslylowerinEPOgroupthaninische

5、mia／reperfusiongroup．(3)StructureofneuroninEPOgroupismorecontactthanthatinischemia—reperfusiongroup．Conclusion：EPOcanprotectmitochondrialfunctionandstructurefrombrainischemia／reperfusioninjury．Keywords：erythropoietin，ischemia-reperfusioninjury，mitochondria6缩写词表缩写词英文全称MCAOmiddlecerebralarteryocclusi

6、onEPohdSmlnmlmggNOI。RIEPoRSODMDACISCIMPTPAIFCyt—c△vmerythropoietinhourdaysecondminutemillilitermilligramgramnitricoxideischemia——reperfusioninjuryerythropoietinreceptorsuperoxidedismutasemalondialdehydecerebralischemiastrokecerebralinfarction中文全称大脑中动脉栓塞促红细胞生成素小时天秒分钟毫升毫克克一氧化氮缺血再灌注损伤促红细胞牛成素受体超氧化物歧化酶丙

7、二醛缺血性脑卒中脑梗塞Mitochondrialpermeabilitytransitionpore线粒体通透性转换孔ApoptosisinducingfactorsCytochromeCMitochondrialmembranepotentialCaspase一3Caspase-3IAPsATPDABInhibitorofApoptosisProteinsadenosine—triphosphatediam