黄精皂苷对慢性应激抑郁大鼠5-ht1ar介导的camp-pka-creb信号通路的影响及其可能机制

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1、安徽医科大学硕士学位论文目录英文缩略词照............................................................................................6摘要............................................................................................................8英文摘要......................

2、.............................................................................111前言......................................................................................................152实验材料...........................................................

3、...................................172.1动物................................................................................................172.2药物与试剂....................................................................................172.3仪器与设备........

4、............................................................................183实验方法：...........................................................................................193.1动物分组及给药...............................................................

5、..............193.2大鼠慢性应激抑郁模型的建立....................................................193.3自主活动时间测定........................................................................213.4体重变化测定......................................................................

6、..........213.5悬尾不动时间测定........................................................................223.6糖水摄入量的测定........................................................................223.7cAMP的检测..........................................................

7、.......................223.7.1样本处理…………………………………………………….223.7.1.1血样本处理…………………………………………..223.7.1.2海马、大脑皮质组织样本处理……………………..223.7.2cAMP含量的测定………………………………………….233.85-HT1AR、PKA、CREB的检测.................................................243.8.1取材与制片.............

8、................................................................243.8.2免疫组化方法……………………………………………....243.9统计学处理....................................................................................254.结果....................................................