大鼠青光眼滤过泡瘢痕化模型中mmp-2及timp-2表达的实验研究

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1、青岛大学硕士学位论文大鼠青光眼滤过泡瘢痕化模型中MMP--2及TIMP--2表达的实验研究姓名：明佩佩申请学位级别：硕士专业：眼科学指导教师：王大博20120522ExpressionsofMMP-2andTIMP-2inaratmodelofglaucomafilteringsurgeryAbstractObjectiveThisstudywasdesignedtoevaluatetherolesofmatrixmetalloproteinase-2(MMP一2)andtissueinhibitorsofMetalloproteinase一2(TI

2、MP-2)insubconjunctivaltissuewoundinghealingafterglaucomafiltering‘surgery．Experimentswereperformedinaratmodel．MethodsGlaucomafilteringsurgerywasperformedon60Sprague—Dawleyratsbyintroducingaanteriorchambersiliconecannula．Respectivelyatdays1，3，5，7，14and28afteroperationtheywerekil

3、ledinbatch．TissueWasharvestedfromtheconjunctivalandsubconjunctivaltissue．Onehalfoftheblebtissuesweremadepathologicalsection，andthenwereH—Estainedandobservedunderlightmicroscope．Theothersofthetissuesweredetectedbysemi-quantitativewesternblotanalysis．Results1．Followingtlfecannula

4、GFS，well—elevatedfilteringblebsformedandgraduallyfailedoverthecourseof7to17days，withameantimetocompletefailurewas12days．2．Histologicalexaminationshowedthatinflammatorycells，fibroblasts，vasodilatationandcollagenoccurredintheprogressofscarringformation．3．Westernblotresults：MMP-2W

5、asdown-regulated1dayaftersurgery,up—regulated3daysaftersurgeryandobservedwithapeakat7daysaftersurgery,andthenitpersisteduntil28Idays．Thedifferencewasstatisticallysignificant(p<0．01)．TIMP-2wasup—regulated1dayaftersurgeryandobservedwithapeakat5daysaftersurgery,andthenitpersistedu

6、ntil28differencewasstatisticallysignificant(p

7、t：MingPeipei(Ophthalmology)DirectedbyProf．Da—BoWangKeywords：Glaucomafilteringsurgery(GFS)；Matrixmetalloproteinase-2；Tissueinhibitorofmetalloproteinases一2；Scarring引言青光眼是主要是以眼压升高导致视神经和视野缺损为主要特征的一种致盲性眼部疾病。眼压水平是青光眼视神经损伤的主要影响因素之一。世界卫生组织2005年公布数据表明，全球大约有7000万青光眼患者，估计到2020年青光眼患者将达到800

8、0万，其中双目失明的患者将至11lO万人n1。目前青光眼滤过手术是公认的药物治疗控制眼压不理想的主要治疗方法