analysis of proteins and gene sequences：蛋白质和基因序列分析

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1、ANALYZINGGENEANDPROTEINSEQUENCESAngeliqueBosseMontgomeryBlairHighSchoolabosse@mbhs.eduTableofContentsPageIntroduction1Scenarios1Activity#1BLAST3Activity#2TaxonomicInformation3Activity#3GeneSequenceSearch4Activity#4RestrictionEnzymeAnalysis5Activity#5FindSimilarProteinSequencesfromVar

2、iousSpecies5Activity#6ComparetheAminoAcidSequencesFromTwoDifferentSpecies6Activity#7PhylogeneticTreeDiagrams7Activity#8PredicttheSecondaryStructureofaProtein8Activity#9FindInformationonAnyEnzyme8Activity#10BiochemicalPathways9Activity#11GeneticDiseasesAssociatedwithaProtein9Activity#

3、12FindingMutationsAssociatedwithaProtein10Acknowledgments10IntroductionThefollowingactivitiesintroducestudentstotheuseofdatabasesasaresourceforgeneandproteininformation.Theseriesoftwelveactivitiescanbedoneinorder,inparts,orcanbemixedandmatchedtosuittheobjectivesoftheunit.Theteacherma

4、ytailortheactivitiestothelevelofstudents.Advancedstudentsmaysolvecomplex,open-endedproblemsandworkattheirownpace.Otherstudentsmayworkinpairstocompletefocusedtasks.Whateverthelevelofstudent,usinggeneandproteindatabasesandotherInternetresources,studentscanperformmanytypesofanalysesonth

5、esequences.Studentsmaysearchvariousdatabases,investigatetaxonomy,andperformanumberofanalyses.Forexamplestudentscancomparehumangene/proteinsequencestootherspecies’sequences,simulaterestrictionanalysis,predictsecondarystructureofaprotein,andgeneratephylogenetictreediagrams.Thestudentsm

6、ayalsofindinformationonenzymes,biochemicalpathways,geneticdiseases,andmutations.ScenariosBelowaresomeideasofhowtoapproachthesebioinformaticsactivities:1.Phenylketonuria:Thisdisease,alsoknownasPKU,iscausedbyaninbornerrorofmetabolism.PKUisautosomalrecessive,whichmeansbabiesbornwithPKUh

7、avetwoabnormalcopiesofthegenephenylalaninehydroxylase.Thisgenecodesforanenzymethatconvertstheaminoacidphenylalaninetotheaminoacidtyrosine.Defectiveenzymeresultsinabuild-upofphenylalanine.Thehighlevelsofphenylalanineresultinmentalretardation.Thediseasemaybedetectedatbirththroughtherou

8、tinenewbornb