肠道病毒71型的感染对细胞内钙离子分布的影响

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1、肠道病毒7l型的感染对细胞内钙离子分布的影响TheeffectofInfectionofEnteroviruse71onthedistributionofcellCa2+Abstract：Enteroviruse71(EV71)hasbeenrecognisedasafrequentcauseofepidemicsofhand-foot-and—mouthdisease(HFMD)associatedwithsevereneurologicalsequelaeinyoungchildrenunder5yearsof

2、age，includingasepticmeningitis，brainstemand／orcerebellarencephalitis，andacuteflaccidparalysis(AFP)ThecardinalsymptomofHFMDisthatPatientshadsymptomsoffever，palmorsoleappearedherpes，oralmucosaappearedinherpesandaccompaniedbythefeelingofpain．Theneurologicalcompli

3、cationsofEV71infectionmayoccasionallycausepulmonaryedemaordeath．SincethefirstcaseofEV71infectioninCaliforniain1969，morethan10worldwidereportsofoutbreakshavefollowed，especiallyinAsian-Pacificregion．SoitisraisingconcernthatboththeprevalenceandvirulenceofEV71mayb

4、eincreasing．Enterovirus71(EV71)belongstothehumanEnterovirusAspeciesoftheEnterovirusgenuswithinthefamilyPicomaviridae．Virionsconsistofanon-envelopedcapsidsurroundingacoreofsingle—stranded，positive—polarityRNAapproximately7．5kbinsize．Theviralcapsidisicosahedrali

5、nsymmetryandiscomposedof60identicalunitseachconsistingofthefourstructuralproteinsVP1．VP4．Thesingleopenreadingframe(ORF)encodesapolyproteinof2194aminoacids．AndthepolyproteiniscleavedintothreeregionsP1，P2andP3．P1encodesfourviralstructuralproteinsVP1一VP4；P2andP3e

6、ncodesevennon-stmctur-alproteins2A—Cand3A—D．Thefunctionsofthe1individualEV71proteinsarethoughttobeidenticaltothatdeterminedforEV71．Ca2+isaubiquitouscationandauniversalsignalingmoleculefoundintheintracellularandextracellularenvironmentofvirtuallyalleukaryoticce

7、lls．Itplayscrucialrolesincellularprocessesincludingcellgrowth，celldeath，cellmotility，andmusclecontraction．ItWasreportedthatmanyvirusescouldaffectonthedistributionofcellCa2+．Accordingtoourresearch．EV71couldinfluencethedistributionof4垒堕望壁intracellularcalciumions

8、．WefoundthatEV7linfectionresultedinthereductionofca2+contentinendoplasmicreticulum(ER)whereasraisedthecytosolicCa2+concentrationandtheinfuxofCa2+inmitochondria．TheinfuxofCa2+inmito