河川沙塘鳢人工育苗的关键因子分析与鸭绿沙塘鳢线粒体dna基因组分析

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1、些!坚箜!blastulaperiodtothegastrulaperiodbeforehatchingandtheycouldnotgrowregularlytothehatch．Withinthetemperaturerangeof15-30。C，withtherisingoftemperature．thetimeDpotamophliaembryosneedtogrowtoeachperbdaregettingshorterandshorter,butwhenthetemperaturereaches30。C，man

2、yembryoshavedeformityinthedevebpmentanddieatthesegmentationperiodinsuccession．Whenthetemperatureis27。C，althoughembryosdevelopsquickly,thedeformityrateisrefativelyhigh，reaching9．7％；whenthetemperatureisbetween18。Cand24。C．onlyfewembryoshavemalformation．Throughthecomp

3、arisonofhatchingratesatdifferenttemperatures，itfindsthatthehatchingrateisthehighest(83．7％)at24。C：thehatchingrateisalsoquitehigh(above70％)at18and21℃；thehatchingrateisrelativelybw(40％-60％)at15。Cand27。C．Thehatchingratereachesthelowest(onlyl3％)at30。C．Synthesizingfacto

4、rslikeincubationtime，hatchingrateanddeformityrate，21℃tO24。Cistheoptimumtemperaturerangeforthedevelopmentofppotamophliaembryos．3．ThepreventionandtreatmentforsaprolegniasisofDpotamophl&zygotesChooseDpotamophliazygotesthatnormallydevelopandtreatthemrespectivelywith2．

5、0，4．0，8．0，16．0and32．0mg／LKMn04solution,C16H18C1N3SsolutionandNaCIsolutionandobservethedevebpmentsituationofzygotes．Throughtheexperiment,itfindsthatwhentheconcentrationofC16HlsCIN3Ssolutionrangesfrom8．0-32．0mg／L，thesaprolegniasisinfectionrateofembryosisbelow10％，；wh

6、entheconcentrationofNaCIsolutionrangesfrom16．0—32．Omg／L，theembryosmildewrateislowerthan10％；whentheconcentrationofKMn04solutionrangesfrom2．0-32．Omg／L，theembryos’mildewrateisalwayshigherthan40％．Therefore，itsuggeststhatC16H18CIN3andNaCIsolutioninhighconcentrationcoul

7、dbotheffectivelycontroIthesaprolegniasis．butthehatchingrateofDpotamophl&zygotestreatedwithNaCIsolutioninhighconcentrationisbelow20％．ButwhentheconcentrationofCl6H]sCIN3solutionisbetween4．0and8．0mg／L，thehatchingrateisabove80％．Synthesizingtheinfectionrateandhatchingr

8、ate，C16HlsCIN3hasthebesteffectonthepreventionandtreatmentforsaprolegniasisofD．potamophliaembryos．4．TheeffectofsalinityonthesurvivalandgrowthofjuvenileDp