直肠癌抑制性消减杂交片断在直肠癌组织与正常直肠组织差异表达分析

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1、frequentlywithsexreversal．PIGRThepoly—Igreceptorisexpressedonseveralglandularepitheliaincludingthoseofliverandbreast．I￡mediatestranscellulartransportofpo]ymericinmunoglobulinmolecules．ItiSamemberofthei姗unoglohulinsuperfamily．Thereceptorhas5unitswithhomologytothevariable(v)unitsofimmuno

2、globulinsandatransmemhraneregion．whichalsohassomehomologytocertaininmunoglobulinvariableregions．EEFI—deltageneencodesasubunitoftheelongationfactor～lcomplex，whichisresponsiblefortheenzymaticdeliveryofaminoacyltRNAstotheribosome．Ashasbeenmentioned，thefourgeneshelptotranscriptionandprotei

3、nfunctionandinmunityactions．weanalysetheirexpressioninhumanrectumadenocarcinomaandnormalrectumtissue．inordertodiSCUSSthemechanismofHumanRectumAdenocarcinomas．Method：MonitortheexpressionofHSPEI、SOX9andPIGRinpatientswhohavehumanrectumadenocarcinomathroughRT—PCRandReal—timePCR．Detecttheex

4、pressionofEEFl--deltainpatientswhohavehumanrectumadenooarcinomathroughReal—timePCRandlineblot．Results：1．IthasbeenshowedthatexpressionofthefourgenesinadenocarcinomatissuehasprominentdiffereneecomparedwithnormalrectaltissuebythetechniqueofReal--timePCR，HPSEIandEEFl一deltashowahigherexprss

5、ioninadenoeacinoma：2．IthasbeenshowedthatHSPEIandPIGRhasprominentdifferencecomparedwithnormalbythetechniqueofPT—PCR．whileresult7ofSOX9showsthsttheyhavenodifference：3．IthasbeenshowedthatPIGRhasnodifferencecomparedw1thnormalbythetechniquelineblot·Conclusion：Ithasbeenshowedthattherearehigh

6、erexpression，1boutHPSElandEEFl一de]taintheprimaryadenoearcinomatissuethaniothenoHIlalrectumtissue．AndatthesaIIletime，therearelowerexpresslonaboutSOX9andPIGRintheprimaryadenocarcinomatissuethani“th。aormalrectumtissue．KeyWords：rectumadenocarcinomasHSPElSOX9PIGREEFlDgenereV。。8etranseriptas

7、e—PCRReal—timePCR．和面结直肠癌是常见的消化道恶性肿瘤之～，占消化道癌的第二位，对人类危害严重。多年来，国内外学者对结直肠癌的发病因素进行了广泛探索，认为结直肠癌是多步骤、多阶段发展的：结直肠癌发生包括癌基因的激活，抑癌基因的失活，错配修复基因的突变。目前主要发现抑癌基因p53、DCC、APC、MCC，癌基因K-ras、c-myc以及人类错配修复基因hMLHl、hMSH2、hPMSl、hPMS2与结直肠癌发病相关。结直肠癌中p53突变率约70％，突变热点位于蛋白质175位氨基酸残基⋯。在结直肠癌中，p53突变是APc突变后的继发

8、生事件；DCC在