c-met与c-src蛋白在人非小细胞肺癌a549细胞系放疗前后表达与活化水平变化的分析

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1、e-Met及c-Src蛋自在人非小细Theresearchofexploringthechangeofc—Metandc—SrcexpressionandactivitytoradiationinhumannonsmallcelllungcancercelllinesA549cellcaneercelllinesI导论文课题起止时间：至Q!至生垒旦=2Q羔兰生垒旦论文完成时间：至Q兰圣生垒旦中国医科大学(辽宁)2013年5月·英文论著摘要·Theresearchofexploringthechangeofc--MetandC—

2、SrcexoresslonandactivitytoraaliationinitUinan^●l·●-·l●·■●lnonsmallcelllungcancercelllinesA549ObjectiveTodetectthechangeofc-Metandc-Srcexpressionandactivityinhumanlungcancercellsafterradiationinordertoexploretheefficacyofthemandthemechanism．MethodsWeusedhumanlungcanv

3、．A：rcelllinesA549invitroasresearcho场ect．WesternBlotassayWasusedtoevaluatethechangeofc-Metandc-Srcexpressionandactivityinhumanlungcancercellsindifferenttimepoimbeforeandafter8Gyradiation．ResultsWesternBlotresultsshowedthatthephosphorylation·-C··Metreachthepeak2hoursa

4、fter8GyX—rayactingonA549celllines．It’S4．41timesasmanyastheexpressionofbeforeirradiation．Thenitdecreasedgradually．Itmarkedtheincreasedactivationofc-Met．Theexpressionofnon-phosphorylationc—Metisundifferentiated．Thephosphorylation-c-Srcreachesthepe出4hoursafter8GyX-raya

5、ctingonA549celllines．Ithasincreased2．99timesasmanyasthosewhodidnotreceiveradiation．Thenitdecreasedgradually．Itmarkedthemcreasedactivationofc—Met．Theexpressionofnon-phosphowlationc-Metisundifferentiated．ConclusionTheactivationofc．Metandc-Srcinhumannonsmallcelllungcan

6、cercellshasmarkedchangesbeforeandafterirradiation,hintingthatc—Metandc—Srcproteinparticipateinthecellsignalingafterradiation．Themechanismofthismaybethatthe3relatedupstreamsignalingpathmakesthemactivate,afterthattheyareinvolvedintheactivatedsignalpath．Theresultsofthi

7、sresearchprovidefoundationforfurtherexploringthemechanismofradiosensitizingeffectinnonsmallcelllungcancerandreferenceforwhethere-MetOrc—Srcinhibitorcanbeusedtopreventtheradiosensitizingeffectinclinicalapplicationforthepatients．KeywordsCarcinoma,Non-Small-CellLung；Ra

8、diotherapy；Proto—OncogeneProteinsc-metProto—OncogeneProteinspp60(c—src)4·英文缩略语·5·论文·c柏t及c-Src蛋白在人非小细胞肺癌舾柏细胞系放疗前后表达及活化水平变化的研究言行吉刖葺肺癌是我国发病率和