欢迎来到天天文库
浏览记录
ID:32844927
大小:5.57 MB
页数:44页
时间:2019-02-16
《扶正抗癌方对h22移植瘤小鼠e-cadherin和mmp-2表达影响的实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、陕西中医学院硕士学位论文扶正抗癌方对H22移植瘤小鼠E--Cadherin和MMP--2表达影响的实验研究姓名:唐洪成申请学位级别:硕士专业:中西医结合临床指导教师:陈光伟201204摘要⋯1111IIIrIrLIIIIlUlY2109692目的:本实验通过对H22移植瘤小鼠模型的建立,采用免疫组化法检测小鼠灌服扶正抗癌方后肝癌组织及癌旁组织中E-cadherin和删P一2的表达,进一步探讨扶正抗癌方抑制肝癌侵袭转移的作用机制。方法:将85只小鼠中提取15只作为空白对照组,剩余70只小鼠采用H:。腹水型瘤株移植瘤小鼠模型造模,将造模成功的小鼠
2、纳入60只,随即分为荷瘤对照组、替加氟组、扶正抗癌方组、扶正抗癌方+替加氟组4组。每组15只。各组小鼠分别给予相应实验药物灌胃,除扶正抗癌方+替加氟组第l天及第7天采用两药共同灌胃以外,其余各组灌胃每日一次,连用14天药。每日观察小鼠一般情况、体重及死亡情况并做详细记录,第15天将小鼠脱臼处死,测量癌组织、免疫器官的重量,计算抑瘤率、胸腺指数、脾脏指数,并采用免疫组化法检测肝癌组织及癌旁组织中E.cadhefin和MMP.2的表达。结果:1.扶正抗癌方组、替加氟组及扶正抗癌方+替加氟组荷瘤小鼠瘤体重量明显小于荷瘤对照组(P<0.01);扶正抗
3、癌方组与替加氟组组间比较无显著性差异(P>0.05)。2.扶正抗癌方组及扶正抗癌方+替加氟组免疫器官(胸腺、脾脏)指数高于替加氟组(P<0.01);扶正抗癌方组与扶正抗癌方+替加氟组组间比较无显著性差异(P>0.05)。3.扶正抗癌方组、替加氟组及扶正抗癌方+替加氟组小鼠肝癌及癌旁组织中E—cadherin阳性表达率显著高于荷瘤对照组(P<0.01)。4.扶正抗癌方组、替加氟组及扶正抗癌方+替加氟组小鼠肝癌及癌旁组织中MMP.2阳性表达率低于荷瘤对照组(P4、疫功能,改善小鼠的生存质量,其抑制肝癌侵袭转移的作用机制与提高E.cadherin及减少MMP.2的表达水平有关。关键词:扶『F抗癌方;H::腹水型肝癌:E-cadherin:MMP-2。TheFuZhengKangAiFangH22transpIantedtumorsinmiceExperimentaIstudyontheef{ectofE-cadherinandMMP一2expressIonAbstractObjective:Inthisstudy,throughtheestablishmentofH22xenograftmousemod5、el,usingtheimmunohistochemicalmethoddetectionmicefedwiththeFuZhengKangAiFangHCCtissuesandadjacenttissuesofE-cadherinand删P一2expression,andfurtherexploreFuZhengKangAiFanginhibitionoflivercancerinvasionandmetastasismechanism.Method:toextract15ofthe85miceascontrolgroup,theremai6、ning70miceH22ascitestumorcellsxenograftmousemodeltomodel,themodelwassuccessfulinmiceintothe60,thendividedintothechargeThetumorcontrolgroup,tegafurgroup,FuZhengKangAiFanggroupFuZhengKangAiFang+tegafurgroup.(N=15).Eachgroupofmiceweregivenanexperimentaldrugorally,FuZhengKangAi7、Fang+foronedayofthefluoridegroupand7daysusingthetwodrugsotherthanthecommongavage,andtheremaininggroupwasgivenonceaday,onceevery14daysofdrug.Dailyobservationofmiceingeneral,bodyweightanddeath,andadetailedrecord15days,themicewerekilled,themeasurementofcancertissue,theweightof8、immuneorgans,theinhibitoryratewascalculated,thethymusindex,spleenindexbyimmunohist
4、疫功能,改善小鼠的生存质量,其抑制肝癌侵袭转移的作用机制与提高E.cadherin及减少MMP.2的表达水平有关。关键词:扶『F抗癌方;H::腹水型肝癌:E-cadherin:MMP-2。TheFuZhengKangAiFangH22transpIantedtumorsinmiceExperimentaIstudyontheef{ectofE-cadherinandMMP一2expressIonAbstractObjective:Inthisstudy,throughtheestablishmentofH22xenograftmousemod
5、el,usingtheimmunohistochemicalmethoddetectionmicefedwiththeFuZhengKangAiFangHCCtissuesandadjacenttissuesofE-cadherinand删P一2expression,andfurtherexploreFuZhengKangAiFanginhibitionoflivercancerinvasionandmetastasismechanism.Method:toextract15ofthe85miceascontrolgroup,theremai
6、ning70miceH22ascitestumorcellsxenograftmousemodeltomodel,themodelwassuccessfulinmiceintothe60,thendividedintothechargeThetumorcontrolgroup,tegafurgroup,FuZhengKangAiFanggroupFuZhengKangAiFang+tegafurgroup.(N=15).Eachgroupofmiceweregivenanexperimentaldrugorally,FuZhengKangAi
7、Fang+foronedayofthefluoridegroupand7daysusingthetwodrugsotherthanthecommongavage,andtheremaininggroupwasgivenonceaday,onceevery14daysofdrug.Dailyobservationofmiceingeneral,bodyweightanddeath,andadetailedrecord15days,themicewerekilled,themeasurementofcancertissue,theweightof
8、immuneorgans,theinhibitoryratewascalculated,thethymusindex,spleenindexbyimmunohist
此文档下载收益归作者所有