小麦叶片对cd、hg和1，2，4-三氯苯胁迫应答的蛋白组学研究

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1、工艳；41麦吐丘盟笪：Hg塑!量，垒：三氢茎胁迫廑签的蛋自组堂研究三ProteomicStudyf．0rwheatleaVesrespondingtoCd，HgandTCBStresSSupervisor：Pro￡GeCai-liIlPr0￡WangYu-10ng(Y曲g出ouU11iV，姆icCollege．‰g出ou，225009)AbstraCtProteomicswmchmaⅡllyanalysesproteiIlsonalargescaleisoneofmemosthport锄tf．acetSmme咖dyoff

2、．unctionalgenonle．EstablishmentofproteorIletedmicalplatf．onnmakesitpossibletos印arate砸ldidenti矽Wheatproltejnswi吐lmgh岫ghout．TheainloftlliseXpedmentistomestig如此e肫Ctofc础Ili啪(Cd)，merCu巧㈣and1，2，4．tricblor01嬲e(TCB)stressonproteomeiIlwheat(洲．Y，mgMail3)leavesbyuS通g也eprote

3、omicteCl证ques(mlu曲培2-DgeleleCtrophoresis，Massspec仃ometric锄mysis，觚dprotemdatab嬲esearch)，so弱t0Comprehendmem01eCularb嬲isofCd，Hg觚dTCBt0)【icity，锄dmeresponsepr0Cessesofmleatt0Cd，HgaIldTCBs仃ess．(1)TCB，Cd，Hg妣ss尚bitedseem吨gro、讹，1edt0、伽erdefic地arldcalISedlipidper0Ⅸidationi

4、nⅡleseedlm铲．(2)Theresultsshowed廿1atTCBandheavymetal(Cd，Hg)s臼．esshadsignijficanteffeCtongloalproteomehl、ⅣheatleaVes．Amlou曲，meexpressionofa1铀驾ermberofproteillsi11Y如gmail31eaveswereeimeri1：lllibitedorirlducedbyTCBandCds臼．ess．皿leaIlalysisof日1ecateoryandf．吼ctionof廿les

5、tressiIlducibleproteionsdemons仃atedmatdia’erent1(indsofresponeswereproducedi11meleavesinresponSet0TCBandCdstress．Themdllctionofmethjonines”nhasesandACCsylltllasepromotedmetllion抽emetab01icp抽waya11dactivaltedme廿lylcycl；e，andw弱ablet0proVideamemyl酽ouptomanyme协．bolit

6、es洫cludingimportantcompoundsⅥ1derCd觚dTCBs仃ess．Theinductionof14．3．3．1ikeprote访，s嘶ne／廿玳onmeprotemphospha‰e2Aregulat0巧sub嘶tandPAS／PACsenSorprote洫haSⅡleroleofmcreaSingorregulatingproteinphospho巧lation．The0ver．expressionofmolecularchaperoneb洫dillgproteill∞iP)锄dheatsho

7、ckprote访werebeneficialtomaintainmeno姗aItllree-dimensional鼬mctureofprotehls．The逾血lctionofSEr-donlaincontajmngproteill(Group40)and耻biscoa肚．V舔ereducedt}leproteolyticsusc印tibilitytorubiscoproteiIl，alldregulatedtheRubiscoactivity．nei11duction0rover-eXpressionofvacu01孙

8、邯_enA’碍ases0rs删tSwerebeneficialt0obtajnt11eo衄1alaCidicpHforvesicletra缅曲培，hydrolaseactiVating，觚dmenlbraJletraIlsportillg，a11ditmaybeals0f．aVorableforaCcumulatmg