【7A文】QTRAP 4000 Operator Training(全英文培训教程).ppt

《【7A文】QTRAP 4000 Operator Training(全英文培训教程).ppt》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、QTRAP4000OperatorTrainingWhatisLC/MSLC:LiquidChromatographyMS:MassSpectrometeraninstrumentthatproducesionsandseparatestheminthegasphaseaccordingtotheirmass-to-chargeratio(m/z)Generatedesolvatedions(Sources)Transferionsintomassspectrometrevacuum(InterfaceRegion)Massanalyzeions(Quadr

2、upolesinvacuumcondition)Detections(Detectorinvacuumcondition)ProcesstheData(Software)ProcessNeededtoGenerateaMassSpectrumTurboVIONSOURCEHousingIonSourceProbesInterfaceFixedSprayerGeometryPlug&PlayInterchangeableTurboIonandAPCIProbesAPIAnalyticalDomainsNeutral101102103104105Molecul

3、arWeightIonicAnalytePolarityIonSprayAPCIGC/MSTurboIonSpraySource:ISVoltage-DeclustersandgeneratesionsIS-VerypHdependentforchargingofmoleculeNebulizer&TurboGas-Declustersandde-solvatesIonsTurboIonSpray(cont)ModifiersOrganicacids(e.g.formic,acetic)promoteionizationofbasiccompounds(sp

4、3N-containing)AdductFormation-Neutralcompoundscontainingnucleophiliclonepairs(sp2N,sp3O)canbeionizedbycationizationwithalkalimetalorammoniumions.RecommendedBuffers-Ammoniumformateoracetateareat(2-10mMoptimum,canseesuppressioneffectsover20mM),.1%FormicAcid,(.1%TFAOK)TurboIonSpray(co

5、nt)CompoundsThatCauseSensitivitySuppressionSaltscaninterferewithionizationandcanclustertocomplicatespectrum(butalsoaidinidentification)StrongbasesorquaternaryaminescaninterferewithpositivemodeanalytesAcids-SulfuricandTFAinterfereinnegativemodeexperiments.DONOTUSEPHOSPHATEBUFFERSNon

6、-CovalentDimersinIonSprayDimerSignal=(MW*2)+1onecauseofnon-linearityathighconcentrationsAPCIProbeHeatedNebulizer(cont.)AtmosphericPressureChemicalIonization(APCI)APCIutilizescoronadischargeAPCIisa“three”stepprocess:1)Needleathighvoltageionizesnebulizinggas(airornitrogen)formingprim

7、aryions2)Primaryionsreactimmediatelywithsolventmoleculesformingreagentions3)Reagentionsreact(byprotontransfer)withanalytemoleculesforming(M+H)+inpositiveionmodeor(M-H)-innegativeionmodeHeatedNebulizer-APCIBuffers:Buffers/modifiersnotrequiredforionizationVolatilebufferstoleratedupto

8、50mMVerypolarmodifiersmayr