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1、Protocolofthereal-timePCRfordetectinggeneticallymodifiedplantsandtheirderivedproductsT9JMfMfSiSflF*&*a3.1AmpEraseUNC891uracilN-glycosylase.3.2C,@:C:cycle,t:threshold,S+E~WXBrl%%B~Bl&%~Brl~f$BifEM!!5BrlBCIS~.3.31PC:internalpositivecontrol,me3.4NTC:notemplatecontrol,9fiM
2、R.3.5PCR:polymerasechainreaction,m$$PCR.3.6DNAdeoxyribonucleicacid,li#N@#@&.3.7dNTPadeoxyribonucleosidetriphosphate,li#N&%=@@3.8dATP:deoxyadenosinetriphosphate,jl#NJBW%E%O&.3.9dCTP8deoxycytidinetriphosphate,B!Nl@BZ@@*3.10dG?'P,deoxyguanosinetriphosphate,ENgl$%Z@&.3.11dUT
3、Prdeoxyuridinetriphosphate,j@,#91%jZ%OBgp.3.12bpgbasepair,MBN.3.13~a~maDNAfiCe@.3.14Tris:tris(hydroxymethy1)aminomethane,.Z(E2:!Ffr)aS:!Sm3.15TEIT~~~-C~,EDTA~#~~3.16Lectintl#B#€#.3.17ZEIN:#@B#EB.3.18P~t~~Pcase:phosphoenilpyruvatecarboxylase,~@~~~~~~lf13PB~~.3.19tRNALeu:&
4、#W8#HB*3.2018srRNA:&@&@18s@@#RNA&B$.3.21CaMV35S:35Spromoterfromcauliflowermosaicvirus,%ElTZ#%ZiV%3i$H35SG3Fe3.22NOS:terminatorofnopalinesynthasegene,MlB@igiff&$tsl)SEl%kF.3.23FMV35SZ35Spromoterfromamodifiedfigwortmosaicvirus(cau1imovirusgroup),Z#ZUj-J$j3335S!aS!JT.3.24NP
5、TII:neomycin-3'-phosphotransferasegene,%~~-3'-@@$$ffl%&H.t3.25BAR:phosphinothricinacetyltransferasegenefromBacillusamyloliquefaciens,S/E8Tf-BacillusamyloliquefaciensfTRZB%$$%hBA2&H03.26PAT*phosphinothricinacetyltransferasegene,*Tf#2m$$bAgm.3.27GOX!glyphosateoxidoreductas
6、egene,fgB.$I&BEmSHa3.28CryIA(b):asyntheticgeneencodesthefirst648aminoacids,insecticidal-activeftruncatedproductidenticaltothatofcrylA(6)geneofBacillusthuringiensissubsp,~Z&~j$f-~~&~~0cryIA(b)SEl.3.29Cry3A:B.thuringiensissubsp,Tenebrionis(B.t.t.strainBI256-82B&eE0,i55;##?
7、@&$t~#$~~#~~&(Coloradopotatobeetlelarvae).3.30EPSPS:5-enolpyruvylshikimate-3-phosphatesynthasegene,5-%j3@-3-@@ff&@&&m.&QB#bIlIflPaq%HEl&%%W%%PCRZB&B~8B'IBfi?34$fihk88SN/T1193HdB.#%B#bUIf&q%&H&%%tl$%%PCRZ#&BEMSN/T1194WeSH3%&Tje6BBhS6.1Biia%W%%SBPCR@*,SB&PCREG#%cfrBUA%%g
8、Bt+'JH%%@%BR%W%WB+PCRB@,igt%%a6S@&M%@@&SfiSB%fiH3%.PCRF#W&BRA-~~I@HHW~~A-+M%BB~J%~A#~%SH%-~&B@,~~%~I]~3