p2cp27-dde、β-bhc及其联合对大鼠睾丸支持细胞vimentin、n-cadherin和fshr表达的影响

《p2cp27-dde、β-bhc及其联合对大鼠睾丸支持细胞vimentin、n-cadherin和fshr表达的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、华 中 科 技 大 学 硕 士 学 位 论 文PartⅡEffectsofp,p’-DDE,-BHCandtheircombinationontheexpressionofVimentin,N-cadherinandFSHRinratSertolicellsinvitroObjective:Toinvestigatetheeffectofp,p'-DDE,β-BHCandtheircombinationonVimentin,N-cadherinandFSHRmRNAandproteinexpr

2、essioninSertolicells.Method:Thereal-timefluorescencequantitativePCRandWesternblottngmethodswereusedtotesttheVimentin,N-cadherinandFSHRmRNAandproteinexpressioninSertolicellsinvitro.Results:Comparedwiththecontrol,thelevelsofVimentinmRNAinSertolicellsin

3、creasedsignificantlyinthe10mol/L-BHCgroup.Withtheincreasingdosesofeachpoison,Vimentinproteinexpressiondecreasedgradually.Comparedwiththecontrol,thedescentofallβ-BHCgroupsand50μmol/Lcombinedgrouphavestatisticallysignificantdifference(P<0.05).Withthe

4、increasingdosesofeachpoison,N-cadherinmRNAexpressionwasfirstlyincreasedandthendecreased,witch30mol/Lp,p'-DDE,30mol/L-BHCand10mol/Lcombinationgroupsincreasedsignificantlycomparedwiththecontrol(P<0.05).TheproteinlevelsofN-cadherinin30,50μmol/Lp,p'-

5、DDE,50μmol/Lβ-BHCandallcombinedgroupsweresignificantlylowerthanthecontrol(P<0.05).FSHRmRNAexpressionwasfirstlyincreasedandthendecreased,comparedwiththecontrol,theincreaseof10μmol/Lp,p'-DDEand10μmol/Lβ-BHCgroupshadastatisticallysignificantdifference(P

6、<0.05).Comparedwiththecontrol,themRNAlevelsin50μmol/Lβ-BHCand50μmol/Lcombinedgroupdecreasedsignificantly(P<0.05).Comparedwiththecontrol,theFSHRproteinexpressionin10,50μmol/Lp,p'-DDE,allβ-BHCand50μmol/Lcombinationgroupsdecreasedsignificantly(P<0.05).T

7、herewerestatisticallysignificantdifferencebetween10μmol/Lcombinedgroupandthesameconcentrationofthep,p'-DDEorβ-BHC10华 中 科 技 大 学 硕 士 学 位 论 文separately.30μmol/Lcombinedgroupdecreasedsignificantlycomparedwiththesameconcentrationofβ-BHC(P<0.05).Conclusion

8、:Lowerdosesofβ-BHCcanstimulateVimentinmRNAexpressionandhighdosesofp,p'-DDE,β-BHCandtheircombinationcaninhibitVimentinproteinexpression.Lowerdosesofp,p'-DDE,β-BHCandtheircombinationcanincreasetheexpressionofN-cadherinmRNA,butp,p'-DDE,β-BHCandtheircomb