mif过表达对宫颈癌细胞株siha生物学特性影响的实验研究

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1、山西医科大学硕士学位论文2．转染重组真核表达载体pEGFP．N1一MIF后SiHa细胞中MIF过表达。3．MIF过表达可上调IL．8、NF．1(B、Bcl．2、CyclinDl的表达，并增强SiHa细胞体外增殖、侵袭迁移能力。4．PDTC明显抑制MIF、IL．8、NF．rd3、Bcl．2、CyclinDl的表达，并抑制SiHa细胞体外增殖、侵袭迁移能力。5．MIF与IL．8、NF．r,B、Bcl一2、CyclinDl之间，NF—r．B与IL-8、Bcl-2、CyclinDl之间，IL一8与Bcl．2、CyclinDl之间均存在正相关，推测MIF可能通过NF．KB信

2、号转导通路调节IL．8、Bcl．2、CyclinDl等下游因子的基因表达，而IL一8又可与相应受体结合，激活NF．rd3通路促进Bcl．2、CyclinDl等下游因子的基因表达，从而促进宫颈癌的侵袭和转移。综上所述，MIF过表达可上调IL．8、NF．r,B、Bcl．2、CyclinDl的表达并增强SiHa细胞增殖、侵袭迁移能力，这可能与NF．1(B信号转导通路相关，但有待进一步证实。本实验可能为研究早期宫颈癌浸润转移的分子机制提供线索，为早期基因诊断提供一个有效的标志物。关键词：巨噬细胞移动抑制因子；基因转染；IL一8；NF—KB；细胞增殖山西医科大学硕一Ij学位

3、论文TheeffectsofMIFoverexpressiononbiologicalcharacteristicsofcervicalcarcinomacelllineSiHaABSTRACTCervicalcanceristhesecondlargestfemalemalignanttumors，lymphnodemeta—stasisistheindependentprognosticfactorsinpatientswithcervicalcancer,andisthemainreasonofdeath．Invasionandmetastasisareth

4、emainbiologicalcharacteristicsofmalignanttumor,seekingthemolecularmarkersofinvasivenessandmetastasisofcervicalcancer,toexplorethemolecularmechanismofinvasionandmetastasisincervicalcancer,therewillbecontributedtoearlydiagnosisandtreatmentoftheinte—rventionofmetastasisincervicalcarcinom

5、a．Macrophagemigrationinhibitoryfactor(MIF)isanimportantregulatorofmanyinflammatoryandimmuneresponses，playanimportantroleintheoccurrenceanddevelopmentoftumor．ButthespecificmechanismofMIFpromotetumorcellinvasionandmetastasisanditsimpactonbiologicalbehaviorofcervicalcancercellsbywhatpath

6、waysarenotfullyunderstood．OurpreviousstudieshavedemonstratedthatMIFregulatecellactivityincervicalcancercellsthroughautocrine，promotetheproliferation，differentiation，invasionandmetastasisofcellsthroughparacrine，MIFanditsrelatedfactorsmayplaysanimportantroleinearlycervicalcancerinvasion

7、andtransferationthroughtheERK／MAPK，P13K／Aktsignaltransductionpathway．Previousstudiesinourlabbasedontheexperiments，theuseofrecombinantDNAtechnology,mediatedgeneMIFtransfectedintotheSiHacellsofhumancervicalcancer,detectionoftheexpressionofMIFanditsrelatedfactorsandinfluenceoncellprolife

8、ratio