缺氧条件下vecs对nscs增殖、分化和凋亡的影响

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1、英文铀娶僮本赣久学硕十学僚毕业论文AbstractOhjecfive：InvitrostudiesofhypoxicVECsNSCsproliferation．differentiationandapoptosis，ToVECsandNSCswereculturedtransplantationinthetreatmentofhypoxic—ischemicdiseasesprovideascientificbasis．Methods：1，thefirstthreedaysofnewbornratsbrainhippoeampusneuralstemcells，using

2、immunohistochemicalmethodstoidentifytheneuralstemceils．2，RetrieveHealth～Wistarratbrainculturedbrainmicrovaseularendothelialcellsusingimmunocytochemicalmethodsoftheiridentification．3，promotionofpurificationofbrainmicrovascularendothelialcellswithO．25％trypsindigestionsolutionintosinglecells

3、aftervaccinationinantanberofpoly一1一ysinecoatedglassonthecover．InoculatedwimthreegenerationsoftrypsindigestionofneuralstemcellstOcoverageratioof10to1completelynervecellsCO—culturedmedium．4，paraffinoilwithsterilesugarcoverageincellcultureliquidsurfacepreparationhypoxiasugarsolution．Controlg

4、roups：normalneuralstemcellsat3TC，thevolumefractionofO．05％C02，saturatedhumidityculture；Atotalofhypoxictraininggroup：atotalofcellsculturedfor3days，theculturemediumWaSsuckedoutbythesterileHank’Sbuffercleansing2nd．Hypoxiasugarsolutiontoacommonroleafter4hand28hforfluid，withPBSbufferwashedthree

5、times，othernervecellscompletelymedium，cellsplacedinanincubatorreoxygenationculture；Hypoxiaaloneneuralstemcells：fromthetraditionalthree—generationneuralstemcellswithsterileHanksbuffer2ndaftercleaningmethodsame．5，fluorescenceimmunohistochemicalidentificationunderhypoxicconditionsneuralstemc

6、ellproliferationandapoptosis，Immunohistochemicalstainingidentifiedunderhypoxicconditionsneuralstemcelldifferentiation．Resuils：1，NSCsandVECscultureandidentification：theprimaryseparationNSC4dsinglenervecellhyperplasiaoftheball，AfterpaSsage，nestinimmunofluorescencestaining，cellswerepositives

7、ignal；primaryculturedbrainmicrovascularendothelialcells6—8dbeardedbottle，orpolygonalcellswereflatspindle，nuclearoval．FactorVIIIrelatedantigen．2，thegroupofneuralstemcellsformoflightmicroscopefeatures：thecontrolgroupNSCssomasatiatedandstrongdioptresurroundedbyaglowdis