牡丹、芍药组间远缘杂交花粉萌发与 胚胎发育的形态学分析

牡丹、芍药组间远缘杂交花粉萌发与 胚胎发育的形态学分析

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页数:46页

时间:2019-02-01

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1、河南农业大学硕士论文wassimil《萄矿show占a⋯6葫茹i&矗谳ai畿稀蠢篱谳鬻;;巯精攀;辩=螽攀stucture:Thesphericalprotrusionsonthestigmasurfaceofthreemaleparentwaslesserandmoreobviouswhilethesphericalprotrusionsoffemaleparent。Fen如nn’Wasbiggerandmorevague.4ilnthecrOSSofPlacfiflora’Fenyunu’×Postii’Fengdanbai’地methodofdelayedpollinatio

2、nandtherepeatedpollinationhadbypassedthepre—fertilizationbarriers;Intheprocessoffertilization,germinatedpollensweremuchobservedonstigmas,thepollentubespassedonthetotallengthofthestyles,andprotrudedtotheovaryCanobservedbythefluorescencemicroscope:ForPlactiflora’Fenyunu’×户suffruticosa‘Luoyanghon

3、g’,themethodofmentorpollenpollinationandthedelayedpollinationalesuitableforbypassingpre-fertilizationbarriers,Thequalityandquantityofpollengerminationandgrowthofthepollentubeswerelargest,madpollentubeswhichwereprotrudedtotheovaryCanfoundpartly.ComparedwiththeselfingofPlactiflora’Fenyunu’,thege

4、rminatedpollensandtheyieldofpollentubelengthweremoreless.5。0d~20dafterpollination.twohyb—dcombinationscarpeldevelopednormally,obviouslyincrease,asmallerivoryovule,20dafterthevastmajorityofcarpelOCCUrgraduallyatrophic,ovulegraduallyatrophicinblack,carpelopenat100d,notabortiveovulesdevelopintoda

5、rkbrownseeds;Combinationofselfingafterpollination,carpeldevelopednormally,whenthe40dovuleenlargement,100d,carpelisdarkbrown,seedisdarkbrown.6.Afterpollination,watchthehybridizationcombinationofembryonicdevelopment,foundthat10d~20dafterpollination,thevastmajorityofthefertilizedeggofdisintegrati

6、onoftheabnormalphenomenon,hasbeenobservedinthelaterobservingnormalembryonicdevelopmentprocess;Afterselfpollination,thevastmajorityofnormalembryonicdevelopment,canbeobservedinthecompleteprocessofembryonicdevelopment;Inhybridcombinations.Plactiflora’Fenyunu’×Postii’Fengdanbai’settingpercentageis

7、4.13%.Plact(flora’Fenyunu’×Psuffruticosa‘Luoyangbong’settingpercentageis3.14%.thesellingof尸lactiflora’Fenyunu’settingpercentageis68.34%thecrosscombinationsismuchhigherthanthehybridcombinations.Keywords:Treepeony;:Herbaceouspeony;Distant

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