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1、毕业设计（论文） 题目红掌组培过程中污染分析指导教师系部生化工程系专业生物技术及应用姓名学号 2012年06月25日保存期限：三年保存部门：专业教研室红掌组培过程中污染分析摘要：红掌（拉丁名：Spathiphyllumfloribundum），亦叫安祖花，红鹤芋等，天南星科火烛属。其花色艳丽，花茎挺拔，叶型翠绿美观，是当今世界著名的切花和盆栽花卉之一，市场潜力大，具有极高的观赏价值和经济价值。红掌的繁殖方法通常采用分株繁殖、扦插繁殖和组织培养繁殖。但分株繁殖、扦插繁殖很难满足当前市场的需求，组织培养是红掌快速繁殖的
2、有效途径。由于组织培养中经常出现不同程度的污染问题，造成很大的损失，因此控制污染是植物组织培养苗工厂化生产中重要的技术环节。本文从灭菌方法、内源性污染、继代培养中污染防治等方面，进行了红掌组培快繁过程中污染原因的分析及对策的探讨。关键词：红掌，组织培养，灭菌，污染，防治TissuecultureprocessofpollutionanalysisABSTRACT：Anthurium,alsocalledAndrewFlower,linessuchasflamingo,aFireAraceae.Itsbrightco
3、lors,tallandstraightstem,greenleaf-typeappearance,arewell-knownintoday'sworldofcutandpottedflowers,onelargemarketpotential,withhighornamentalvalueandeconomicvalue.Flowerandraeanumbreedingmethodscommonlyusedrametspropagation,cuttingpropagationandtissueculturepr
4、opagation.However,rametspropagation,cuttingpropagationisdifficulttomeetcurrentmarketdemand,tissuecultureandraeanumFlowerExpressareaneffectivewayofbreeding.Tissueculturebecauseofthefrequentoccurrenceofvariousdegreesofpollution,resultingingreatloss,socontrolofpo
5、llutionareindustrialplanttissueculturevaccineproductiontechnologyintheimportantaspect.Thisarticlefromthesterilizationmethod,endogenouspollutionsubcultureinsuchareasaspollutionprevention,todiscusshowtominimizetheemergenceoftissueinthepollutionproblems.KEYWORDS:
6、Anthurium，tissueculture，sterilization，pollution，preventionandtreatment目录前言···························1第1章培养基的灭菌···················21.1一般培养基的灭菌·······················21.2不耐热物质的灭菌······················2第2章外植体材料的消毒与灭菌·············42.1外植体的消毒与灭菌··················4第
7、3章接种前的准备工作·················53.1器皿、工具的灭菌····················53.1.1器皿与金属器械的灭菌················53.1.2布质制品的灭菌····················53.2培养室的灭菌·····················53.3无菌操作和无菌操作技术················5第4章红掌诱导与继代培养过程中的污染·········7第5章污染的原因和预防措施··············85.1通常污染··········
8、·············85.2内源性污染······················85.3污染原因判断及污染苗抢救···············85.4预防方法和措施···················95.4.1基本方法······················95.4.2经常检查消毒锅的灭菌质量··············95.4.3检查培养容
