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1、werebuildedindifferentgroupsbyanotheroperation.TheobstructivejaundiceSDratswerereceivedleftandmiddlelobehepatectomy(about70%oftotalliver),andalsotheSDratsdrainagedfor7dayswereperformedtheoperation.At0h,4h,12h,24h,48hand72hafterPH,theratsweresuccumbedandthespe
2、cimensofbloodandliverwerecollected.ThelevelsofTBIL,TBAinserumweredetermined,thelevelsofPCNAexpressionweredeterminedbyimmunohistologicaltechnology.RT-PCRwasusedtoquantitatetheCYP7A1mRNAandFXRmRNAandWestern-BlottingtoFXRprotein.Results:(1)Themortalityratesofrat
3、sfollowingPHinIDgroupweresignificantlylowerthaninOJgroupandEDgroup.(2)AfterPH,theliverregenerationratesinIDgroupwashigherthanEDgroupafterin72h(P<0.01),EDgroupwashigherthanOJgroup(p<0.05),ThedifferenceofIDgroupandSHgrouphadnostatisticssignificance(P>0.05).(3)A
4、fterPH，theexpressionofPCNAinallofgroupswereobviouslyincreasedat12h,andgottopeakat24hinIDgroup.TheexpressionofandPCNApeakedat48hinOJgroupandEDgroup,andthepeakvaluesweredelayedandsignificantlylowerthantheIDgroup(p<0.05).(4)AfterPH,thelevelsofTBAinOJgroupwassign
5、ificantlyhigherthanothergroups(P<0.01),andgottopeakat24h.ThelevelsofTBAinED,IDandSHgroupgottopeakat48h,andthepeakvalueswerelowerthanOJgroup(P<0.05).(5)TheexpressionofCYP7A1mRNAinEDgroupwashigherthanSHgroupandIDgroupin0h(P<0.01),thenslowlydecreasedandgotminimu
6、mvalueat48h,theminimumvaluewashigherthanIDgroupandSHgroup(p<0.05),IDgroupandSHgrouphadnostatisticssignificance(P>0.05).(6)TheexpressionofCYP7A1mRNAinEDgroupwaslowerthanSHgroupandIDgroupin0h(P<0.01),thengraduallyincreasedandgotpeakvalueat48h,theminimumvaluewas
7、lowerthanIDgroupandSHgroup(p<0.05),IDgroupandSHgrouphadnostatisticssignificance(P>0.05).(7)TheproteinexpressionofFXRinEDgroupislowerthanothergroups(P<0.01)，then6graduallyincreasedandgotpeakvalueat48h,thepeakvaluewaslowerthanIDgroupandSHgroup(p<0.01).IDgroupan
8、dSHgroupwerehigherthantheothergroupssignificance(P<0.05),andhadnostatisticssignificancebetweenthem(P>0.05).Conclusions:1Bothinternalandexternaldrainagecanreleasebiliaryobstruction,reduces
