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ID:32042354
大小:3.14 MB
页数:62页
时间:2019-01-30
《阿萨希毛孢子菌体外氟康唑诱导耐药的方法分析》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、万方数据第三军医大学硕士学位论文InⅥtroStudyonInductionofResistancetoFluconazoIeiⅡ乃站厅D置pD阳露口s露办村BackgrouⅡdandobjectiVeAbstractmc^D点po,_D,zspp.exsitwidelyinnatllre,comprising50species,ofwhichl6speciescancausehumaninfection.Thenumberofinfectionscausedby乃fc^唧D加,zspp.accountfor10%ofthedisseminated血ngalinfe
2、ctions.乃f幽cl印f)加,z口s砌ffisthecommoncausatiVeagentof乃f幽。印D加九spp.infections,andcanleadtosystemicandfataltrichosporonOsis,especiallyini姗姒mocompromisedandimmIlrIodeficienthosts,suchaspeoplesuff色ringf.romneutropenia,orgaIltransplaIltation,hematologicmalignancies,extensivebumsorAIDSWiththewid
3、espreaduseofa11tiflmgaldmgs,especiallyofazoles,theanti如ngalresistanceofZ口s口Jlliihasbeenincreasin91yreported.ThishasmadeclinicaldoctorsdimculttodealwithtrichosporOnosis,andthedeVelopmentofnewanti如ngalagentsoRenrequirelongperiod.Therefbre,itissignificaIlttoexplorethenuconazoleresistancem
4、echanismsandfind0utnewt盯getsofanti向ngalagents.Theseneedtoestablishastableaz01e—resistantmodelofZ口J口^“ResistantmodelsacquiredbyinductioninVitroo托npresentlowsuccessrateandinstableresistaIlce,soitisimperatiVetoestablishamethodwhichcanacquirestableresistantstrainssuccess如11y.Inthisstudy,we
5、obtainednuconazole—resistantmodelofr口s砌ifwhichwereculturedinmediumscontainingmethylprednisoloneandnuconazole,andevaluatedthesuccessrateaIldstability.Methods1.16clinicalandenviror吼entalstrainsofZ以J砌村weretestedthesusc印tibilityagainstnuconaz01einVitr0byCLSIM27一A3;2.Basedonthesusceptibilit
6、yresults,thenuconazolesensitiVestrainswereinducedresistancetonuconazolebyindiVidualfluconazole,indiVidualmethylprednisolone,andnuconaz01ecombinatedwithmethylprednis010nerespectiVely,comparingwimthecontr01万方数据grOup;第二军医人学硕士学位论文3.InordertoobserVethestabilityofaz01e-resistalltstrains,isol
7、atesfoundtoexhibitFLCresistancewereseriallycultured30timesindmg-freemedium.A佼ereachsubculture,antifungalsusc印tibilitytestingwasperfomled;4.Inordertomeasurethedevelopmentofcross—resistancetootheranti允ngalagents,thestablenuconaz01e—resistalltstminswereperf.ormedMICsassayswithfourantinm
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