甜瓜属双二倍体与栽培黄瓜杂交亲和性-.研究

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1、甜瓜属双二倍体与栽培黄瓜杂交亲和性研究othercombinations，SOthesecrosswereconducivetocreatallotriploidcucumber．ThousandkemelweightalsoreflectedthatthequalityofhybridseedwasbetterwhenC．hytivusasthefemale．Theresultshowedthatthegenotypeofcucumbercultivarsinfluencedtheeffectofhybridization．2．ITSsequenceanalysisofs

2、everalCucumiscropsITSsequenceslength(including5．8s)Was618-640bp谢tllGCcontentof58．7—64．5％indifferentCucumismaterials．Incucumber，thevariableandtheinformativesitesWas105and20，respectively,accountingfor22．01％and4．19％oftotalbases．BasedontheITSsequencesinformationinCucumis，C．hytivushadaclosergen

3、eticdistanceandahighercross-compatibilitywitllthecucumberculfivarsfromChina．InmolecularleveltofLLrtherverifythegenotypeofthecucumbercultivarsinfluencedthehybrideffect．3．PhysiologicalandbiochemicalcharacteristicsofcucumberduringStorageFruitsofNingyunNo．3andNankangNo．1wereusedasthematerialsi

4、nstoragefrom3daysto13daysafterharvesting．Theresultsindicatedthatduringstoragefruitfirmnessdroppedby15．73％forNingyunNo．3and32．74％forNanKangNo．1；fruitweightlossrateWas2．49％forNingyunNo．3and6．17％forNankangNo．1；MDAcontentsignificantlyincreasedby80．15％inNingyunNo．3and102．88％inNankangNo．1；uponst

5、orageforoneweek，theSODactivityofNingyunNo．3remained84．20％higherthanNankangNo．1whereastherespirationrateWas38．29％lowerthanNankangNo．1．Withstorageforlongertime，mostmeasurementsreachedsignificantdifferencethetwocultivars．KEYWORDS：Cucumber；Amphidiploid；Allotriploid；ITS；Hybridizationcompatibili

6、tyIV缩略词表英文缩写英文全称中文全称AmpAFLPAmpicillin胺苄青霉素Amplifiedfragmentlengthpolymorphism扩增性片段长度多态性BLASTBasiclocalalignmentsearchtoolbpcMC咖DNAdNTPIRAPISSRITSMDAMetNBTPCRBasepairCenti·morganCetyl-lrimethyl—ammoniumbromideDeoxyfibosenucleicacidDeoxynucleosidetriphosphateInter-retrotransposonamplifiedpol

7、ymorphismInter-simplesequencerepeatInternaltranscribedspacerMalondialdehydeMethyl—methionineNitrogenbluestetrazolePolymerasechainreactionRAPDRandomamplifiedpolymorphicDNArDNARibosomedeoxyribosenucleicacid基本局域联配搜索工具碱基对厘摩尔根溴代十六烷基三甲胺脱氧核糖核酸脱氧核糖三磷酸逆转座子位点间扩增多态性简单序列重