木薯多倍体育种技术.研究

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1、formulaforbothbeginninggenerationofcassavabudandsubcultureoftuffbudhadaquitegoodcultureeffect．2、111eCassavacallusinductionismoredifficultthanbud．Underthesinglefactorcondition，hormone2,4-Dhadtheinductioncallusability；Underthedoublefactorcondition，MS+2，4-D2．O+6一BA0．05inductionratewasthehigh

2、est,achieved92．86％；ButtheMS+2，4-D2．0+NAA0．1inductioneffectWasthebest,hadahigherinductionrate，moreovertheinducedcalluswasabletoquicklyinductbudunderthesuitableenvironmentalcondition．3、TheradicationofcassavabudWasveryeasy,NAA—inducedeffects，wasbetterthanL蚣andKT,NAAaddon6一BA，thatwastheformul

3、a：MS+NAA2．0+6一BA0．05hadthebiggestinfluencetoradication．4、砀einductionexpedmentthatcassavacallusdifferentiationbudindicated,theMS+2，4一D2．O+NAA2．0coludinducebudsfromcallus．Fromthecassavaleavesofasepsisseedinginducedcallus，thenfromcallusinducedcassavabudwasainnovationofthisexperimental．5、Thef

4、ieldpolyploidyinductionalwaysusesthecolchicinstempointdaubingmethodandsproutgerminatesmarinatemethod，fromtheprocessingcomprehensiveeffectlookedthatthestempointdaubingmethodof60％was—superiortothesproutgerminatesmarinatemethodof40％．6、Usingcolchicininductionintissueculture，itseffectandthefie

5、ldinductiondifferencearenotbig．111eratebothareabout40％．7、Colchicin’SconcentrationrangewasbetweenO．1％一O．5％，moreappropriatetimewaswithin72hours，speciallywheninducedundertissueculture’Scondition，wasmoresuitableforshorttime(below48hours)andlowconcentration(belowO．5％)．8、Underthebigfieldconditi

6、on，thepolyploidmutmioncompareswithitsIVdiploidhadmanydifferences，theconcretemanifestationwasthatlamlnaethickens，thecolorofleavesforthechangesdepth，thedifferenceof’thestomas泌锄dstomadensitybetweenthecassa．vapolyploidandthe1srem冰able，aswellaSthroughthechlorophyllcontentandthestarehcontent’sd

7、etenllinatioIl'polyploidcomparedwithhadaobviousdlfference·9、Identificationofcytologyresultsindicatedthatthepolypl01d∞ottlpchrQmosomenu血berwasobviouslymorethandiploid．Keyword：CassavaTisSUecultureColchicinP。lyploidV缩略语NAA2，4．D6．BAL认KT符号说明英文名称Naphthylaeetieacid2,4-Dich