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1、HANDBOOKOFMODERNBIOPHYSICSSeriesEditorThomasJueUniversityofCaliforniaDavisDavis,CaliforniaForothertitlespublishedinthisseries,gotowww.springer.com/series/7845ThomasJueEditorFundamentalConceptsinBiophysicsVolume1EditorThomasJue,Ph.D.DepartmentofBiochemistryandMolecularMedicineUni
2、versityofCaliforniaDavisOneShieldsAvenueDavis,CA95616,USAtjue@ucdavis.eduISBN:978-1-58829-973-4e-ISBN:978-1-59745-397-4DOI:10.1007/978-1-59745-397-4LibraryofCongressControlNumber:2009921740©HumanaPress,apartofSpringerScience+BusinessMedia,LLC2009Allrightsreserved.Thisworkmaynotb
3、etranslatedorcopiedinwholeorinpartwithoutthewrittenpermissionofthepublisher(HumanaPress,c/oSpringerScience+BusinessMedia,LLC,233SpringStreet,NewYork,NY10013,USA),exceptforbriefexcerptsinconnectionwithreviewsorscholarlyanalysis.Useinconnectionwithanyformofinformationstorageandret
4、rieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdevelopedisforbidden.Theuseinthispublicationoftradenames,trademarks,servicemarks,andsimilarterms,eveniftheyarenotidentifiedassuch,isnottobetakenasanexpressionofopinionastowhetherorn
5、ottheyaresubjecttoproprietaryrights.Whiletheadviceandinformationinthisbookarebelievedtobetrueandaccurateatthedateofgoingtopress,neithertheauthorsnortheeditorsnorthepublishercanacceptanylegalresponsibilityforanyerrorsoromissionsthatmaybemade.Thepublishermakesnowarranty,expressori
6、mplied,withrespecttothematerialcontainedherein.CoverIllustration:Patch-Clampfluorometryofanexcisedinside-outmembranepatch.Printedonacid-freepaperspringer.com/humanaPREFACEOverthepastseveralyears,IhaveorganizedandtaughtacorecourseinModernBiophysicsTechniquesattheUniversityofCalif
7、orniaDavis.Graduatestudentsinbiophysics,chemistry,physics,andengineeringenrollintheclasstosurveythephysicaltechniquesthatscientistsusetostudybiology.Introducingthediverseandcomplexfieldofbiophysicsinanacademicallyrigorousbutin-terestingwayposesdauntingchallenges.Indeed,thecourse
8、hasundergonemanytransforma-tionsandhastriedonma