资源描述：
《培养基的制备与高压灭菌》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、培养基的制备与高压灭PreparationandsterilizationofculturemediumTwo,principle1,medium(culturemedium):culturemediumisakindofmatrixwhichispreparedbyartificialmethodaccordingtothevariousnutrientsneededbythegrowthandreproductionofmicroorganisms・Media:accordingtothecompos
2、itionoftheculturecanbedividedintosemisyntheticnatlira1mediumandsyntheticmedium,medium;accordingtodifferentphysicalproperties,canbedividedintosolidmedium,semisolidmediumandliquidmedium;accordingtothespecialpurposecanbedividedintobasicmediumandenrichedcultu
3、remediumselection,mediumanddifferentialmedium.Thenaturalmedium:isthemaincomponentofnaturalorganicsubstances,suchaspotatoes,cornflour,soybeanflour,beanjuice,beefextract,peptone,serum,thecomponentsthatarenotfullyunderstood,andeachbatchisnotuniform.Itissuita
4、bleforlarge-scaleproductionofmicroorganisms・Thesyntheticmedium:chemicalcompositionofaclearcompoundedmedium.Suchas:GaomediumandCzapekmediumetc・・Itisgenerallyapplicabletothestudyofmicrobialmorphology,nutritionmetabolism,classification,identification,bioassa
5、y,strainselectionandgeneticanalysisinthelaboratory.Thesemisyntheticmedium:Basedonnaturalorganicmatter,appropriatetoaddmediumchemicalingredientsknownprepared・Awiderangeofapplications,suchaspotatodextrosemedium,issuitableforthegrowthofmanymoldsTheliquidcult
6、uremedium:aculturemediumwithagoodliquidstate・Suchassugarfermentationliquidmedium,peptonewatermediumandsoon.Itiswidelyusedintheoreticalresearchandlarge-scaleindustrialfermentation.Thesolidmediuminsolidmediumcoagulantformedbyliquidmediumsupplementedwith.The
7、commonlyusedagar,gelatin,silicagelwithcoagulant・Silicagelisusedtopreparesolidmediaforautotrophicmicroorganisms;formostothermicroorganisms,agaristhemostsuitable,generallyadding1.5-2.5%,suchasbeefpastepeptoneculturemedium,etc・・Thismediumformicrobialisolatio
8、n,identificationandpreservationetc・・Thesemisolidculturemediuminliquidculturemediumwithaddingasmallamountofcoagulantisasemisolidculturemedium,suchasadding0.2一0・7%agarascoagulant・Itisusedtoobservebacterialmovementchar
