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1、文章编号：200804019罗格列酮抑制粘附分子表达减轻大鼠急性心肌梗死再灌注后无再流何泉，雷寒，覃数，马康华（重庆医科大学，附属第一医院心内科，重庆400016）摘要：目的观察罗格列酮对大鼠急性心肌梗死（acutemyocardialinfarction，AMI）再灌注后无再流的影响及其机制。方法45只SD大鼠随机分为假手术组、生理盐水组、罗格列酮组（3mg·kg-1·d-1,共14d），每组15只，后2组大鼠行冠状动脉结扎3h，松解2h建立AMI再灌注后无再流模型。以病理染色法检测无再流面积；化学比色法检测再灌注区心肌髓过氧化物酶（myelepe
2、roxidase，MPO）活性；以免疫组化及RT-PCR方法检测再灌注区细胞间粘附分子-1（Intercellularadhesionmolecule-1，ICAM-1）和P-选择素蛋白及mRNA的表达。结果①（1）罗格列酮组心肌无再流范围较生理盐水组显著减小（P<0.01）；②（2）生理盐水组心肌MPO酶活性、ICAM-1及P-选择素蛋白及mRNA表达水平较假手术组均显著升高（P<0.01）,罗格列酮组上述指标较安慰剂组显著降低（P<0.05）。结论罗格列酮能有效的减小大鼠急性心肌梗死再灌注后无再流范围，其机制可能与抑制再灌注后内皮ICAM-1及P
3、-选择素的表达，进而减少中性粒细胞的粘附及聚积有关。关键词：无再流现象；心肌梗死；罗格列酮；细胞间粘附分子-1；P-选择素中图分类号：R541.4文献标识码：ARosiglitazonereducedtheareaofno-reflowafterAMIwithreperfusioninratsbyinhibitingtheexpressionofadhesionmoleculesHEQuan,LEIHan,QINShu,MAKang-Hua.(DepartmentofCardiology,TheFirstAffiliatedHospital,Chon
4、gqingMedicalUniversity,Chongqing,400016)Abstract：ObjectiveToinvestigatetheeffectandmechanismofrosiglitazoneonno-reflowafteracutemyocardialinfarctionwithreperfusioninrats.Methods45SDratswererandomlydividedintosham-operatedgroup、normalsalinetreatedgroupandrosiglitazonetreatedgrou
5、p(3mg·kg-1·d-1,for14days),15ratsforeachgroup;Toestablishno-reflowmodel,theratsofthelattertwogroupsreceivedcoronaryarteryligationfor3hwithreopeningfor2h;Andpathologicalstainingforno-reflow、chemicalassayforMPOactivity、immunohistochemistryandRT-PCRforproteinsandmRNAexpressionofICA
6、M-1andP-selectininmyocardiumwereperformed.Results(1)Areaofno-reflowinrosiglitazonetreatedgroupwassignificantlyreduced,comparedwiththatinnormalsalinetreatedgroup(P<0.01)；(2)theMPOactivity、proteinsandmRNAexpressionofICAM-1andP-selectininnormalsalinetreatedgroupweresignificantlyin
7、creased(P<0.01),comparedwiththatinsham-operatedgroup,whereasthoseweresignificantlyinhibited(P<0.05)inrosiglitazonetreatedgroup.ConclusionRosiglitazonecaneffectivelyreduceareaofno-reflowafterAMIwithreperfusioninrats,whichmaybeattributedpartlytodownregulationofexpressionofICAM-1a
8、ndP-selectininthemyocardiumafterreperfusion,andfurther
