imspcr检测食品中的单增李斯特菌

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1、Abstract一●————————————————————————————————————————————————————一AbstractListeriamonocytogenesisacommonbacteriuminnature，whichcanoccursbothmh啪ansaIldiIlaIlimals．Listeriosisisaseverediseaseassociatedwithahighcasefatalityrate．SoiffoodscontainListeriamonocytogenes，itisveryimporta

2、nttoadoptscientificmethodstodetecttllem．IMS．PCRmethodcailquicklydetectListeriamonocytogenesinfoods·IMSutilizetheprincipleofantigen—antibodyresponseanddoListeriamonocytogenes'experimentsofspecificityandsensitivity．PCRisthatonesetofprimersWasdesignedaccordingtothesequencesofth

3、elapgene(P60proteincoding)ofLiteriamonocytogenesandconductedPCR；anddoListeriamonocytogenes"experimentsofspecificityandsensitivity·Accordingtoanalyzeandcomparethetwomethods，wesetupIMS—PCRandemploythefonnerpriIIlertodoListeriamonocytogenes"experimentsofspecificityandsensitivit

4、y．AlsoGuobiao(standardmethodofinspection)andRealTimePeR(kit)takeasthecontrolofIMS．PCR,thenanalyzeandcompareIMS-PCRSresults．IMScanquicklyentrapbacteriatoincreasetheminculturefluid(37"C14"--18h)，andhemolyticreactionandrhamnosereactioncanconfirmifitisLiteriamonocytogenes，anddem

5、趾ds3"-'7days；sensitivitycanattain10"7．PCR：ObtainingLiteriamonocytogenesfromfoodstocarryoutenrichmentculturewhichneed3"-5days．ExtractbacterialgenomeDNAtocarryoutPCRanddeserve230bp,whicharriveatresultsfor4hours，itssensitivitycallattain10"5．IMS—PCR：Fromfastcollectingbacteriatop

6、rogressenrichmentculture，thendeselwe230bpafterPCR,itCandetectLisiteriamonocytogenesinaday．Itlessenedthetimeofinspection，atthesametimeitraiseddetectivespecificityandsensitivityofLisiteriamonocytogenes·TheresultsofIMS．PCRagreewithGuobiaoandRealTimePeR(kit)，thenitismoreconvenie

7、nt．fasterandsensitivethanGuobiao；andlowercostthanRealTimePCR(kit)．Sofromeconomyandpracticability，IMS—PCRhasagoodprospecttouseandvaluetOstudy·Keywords：Listeriamonocytogenes,Guobiao，imuno-magneticcelIseparation，PCR。tempIate，ReaITimePCR，IMS—PCRII关于硕士学位论文使用授权的说明论文题目：!鳖二￡￡基捡型盒晶生鲍

8、皇擅奎逝挂菌本学位论文作者完全了解大连工业大学有关保留、使用学位论文的规定，大连工业大学有权保留并向国家有关部门或机构送交论文的复印件和磁盘，允许论文