番茄抗黄化曲叶病基因ty-5r遗传分析及分子标记

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1、ClassifiedIndex：Code：10224Confidential(yes／no)：noNO．：120410276DissertationfortheMasterDegreeGeneticAnalysisandGeneMappingofYe／／owLeafCurlVirusResistantGene咖．5‘T0matoin加一5’．01matoCandidate：YangHuanhuanSupervisor：ProfessorLiJingfuDegreeCategory：MasterofAgricultureCollege：CollegeofHorticult

2、ureFirstleveldiscipline：HorticultureSecondleveldiscipline：OlericultureHarbinChinaJune2015万方数据目录㈣㈣㈣㈣删㈣删0Y2771679目录摘要I英文摘要IIIl引言一11．1研究的目的和意义．11．2国内外研究进展．11．2．1TYLCV的研究进展．11．2．2TYLcv及其传播一21．2．3番茄黄化曲叶病毒(TYLCV)接种及抗性鉴定21．2．4番茄黄化曲叶病主要防治方法．31．2．5抗番茄黄化曲叶病育种一41．2．6分子标记技术51．2．7抗病品种的选育81．3研究内容、技术路线

3、．92材料与方法112．1试验材料l12．1．1番茄材料l12．1．2试验试剂．112．1．3主要仪器与设备．112．2．1试验设计．112．2．2番茄黄化曲叶病毒病抗性鉴定122．2．3DNA提取及抗感池构建132．2．4ty一5基因SSR连锁群的构建及染色体初定位．142．2．5数据统计及连锁距离的测定152．2．6番茄黄化曲叶病毒种质资源的抗病性鉴定153结果与分析173．1遗传规律分析173．1．1番茄黄化曲叶病抗性遗传分析173．1．2各世代发病的株问分布173．2基因组DNA的提取183．3SSR分子标记结果】93-3．1SSR引物的筛选一193．3．2抗、

4、感池的构建．193-3．3F2代单株的SSR标记203．4连锁关系作图20万方数据东北农业大学农学硕士学位论文3．4．1SSR标记的遗传连锁分析．203．4．2连锁关系作图．203．5番茄黄化曲叶病毒病抗病性鉴定．．224讨论．．244．1抗病性鉴定244．2抗病基因的分子标记244．3番茄抗TvLcv种质资源的筛选和品种的选育．255结论26致谢27参考文献28附录36攻读硕士学位期间发表的学术论文万方数据CONTENrSCoNTENTSAbstractinChineseIAbstractinEnglish。······························

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7、·······t··············-········‘21．2．4PreventionMeasureofTomatoYellowLeafCLIrlVirus···-··。31．2．5ScreeningofResistanceSourcesandInheritanceofResistance··41．2．6MolecularMarkerandResistantbleBreeding-”51．2．7ResistantbleBreeding‘··_······-···············-···············-·····