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ID:27735022
大小:1.30 MB
页数:14页
时间:2018-12-05
《嵌入式开发-viral delivery systems》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、. . . . . . . . . . . . . . . . . . . . . . . . . . . .CLONING.. .ViralDeliverySystems. . . . . . . . . . . . . . . . . . . .+.. . .. .GeneDelivery......................................................................................................................
2、.............................. . . . . . . . . . . . . . . . . . . . . . . .2>3VIRALDELIVERYSYSTEMS>CUTTING-EDGEGENEDELIVERY............................................................................................................................Cutting-EdgeGeneD
3、eliveryAssciencemovesfromgenediscoverytounderstandinggenefunction,theneedtostudygene expressioninanativehostisbecomingincreasinglyimportant.Manyofthesehostsaredifficult orimpossibletotransfect,whichmeansfunctionalstudiesmaybelimitedtohoststhateasily acceptDNAusingt
4、raditionaltransfectionmethods.Tosolvethisproblem,viral-basedgene deliverysystems,likeourAAVHelper-FreeSystem,AdEasy™adenovirussystem,andViraPort® retroviralcDNAexpressionsystemhavebeendevelopedforexceptionallyhigh-efficiencygene deliverytoabroaderrangeofhosts.Sohow
5、doyoudecidewhichviraldeliverysystemtochoose?Thetablesbelowcompareourthree viralgenedeliverysystemstomakeiteasytofindthebestsystemforyourapplication.ApplicationLong-TermGeneExpressionTransient,High-LevelGeneExpressionFunctionalCloningAssaysSystemAAVHelper-FreeSystem
6、AdEasy™XLAdenoviralSystemViraPort®RetroviralcDNAExpressionSystemAdvantages+Infectsbothdividingandnon-dividingcells+High-levelproteinproduction+Integratesintohostgenomeforstableexpression+Long-term,stablegeneexpression+Infectsbothdividingandnon-dividingcells+Copynum
7、bercontrolledbymultiplicityof +Unparalleledbiosafetyprofile+HomologousrecombinationinE.coliinfection savesweeksofwork+FunctionallyscreencDNAlibrariesinmammaliancells+PremadelibrariesavailableHOWTOCHOOSETHERIGHTSYSTEMBYAPPLICATIONSystemAAVAdEasyXLViraPortTransfectio
8、nGenedeliveryefficiency>90%>90%>90%~20%Host:Dividingcells++++Host:Non-dividingcells++−−Long-termexpression+−++Transientexpression−+−+High-titervi
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