杀虫剂和植物次生代谢物对飞蝗细胞色素P450基因的诱导表达

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1、-3.2TheanalysisresultsofreferencegenesbygeNorm............................................123.3TheanalysisresultsofreferencegenesbyNormfinder......................................123.4Tissue-specificexpressionpatternsofP450genesfromLocustamigratoria....124D

2、iscussion...............................................................................................................14Chapter3TheinductionofP450genesfromLocustamigratoria.......................171Material.................................................

3、...................................................................171.1Insect.....................................................................................................................171.2Reagents.................................................

4、...............................................................171.3Instruments............................................................................................................172Methods............................................................

5、........................................................172.1Thepreparementofexperimentalmaterials.......................................................172.2ExtractionandpurificationoftotalRNAandsynthesisoffrist-strandcDNAfromexperimentmaterials...........

6、........................................................................................182.3Thechoiceofreferencegenes.............................................................................182.4TheinductionofP450genesfromLocustamigratoria.............

7、.............................183Result........................................................................................................................183.1TheanalysisresultsofreferencegenesbygeNorm............................................183.2The

8、analysisresultsofreferencegenesbyNormfinder......................................193.3TheinductionofP450genesfromLocustamigratoria...........................................203.3.1PestcideinduceP450gen