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1、南方红豆杉种子综合处理过程中内源激素的动态变化（英文）广西植物Guihaia31(3):370—3762011年5月DOI:10.3969/).issn.1000—3142.2011.05.017南方红豆杉种子综合处理过程中内源激素的动态变化张艳杰,鲁顺保,高扞东(1.江西师范大学生命科学学院,南昌330022;2.南京林业大学森林资源与环境学院,南京2l0037)摘要:为揭示南方红豆杉种子内源激素与休眠的关系,采用酶联免疫吸附法(EIIsA)测定了经过层积处理的种皮和胚乳的脱落酸(ABA),赤霉素(GA3),吲哚乙酸(IAA),玉米素核苷(ZR)4种内源激素含量的变化情况
2、.结果表明:种子胚乳中内源ABA的含量随着层积时间的延长而逐渐下降,GA含量增加,IAA和ZR的含量先增加后降低,GA/ABA,IAA/ABA和zR/ABA逐渐增大,休眠随之解除.种皮中内源ABA,GA,IAA和ZR的含量均随着层积时间的延长而逐渐下降.其中GA/ABA的变化较大,因此,南方红豆杉种子休眠的限制因子很可能是ABA和GA的平衡调控作用.关键词:南方红豆杉;种子;内源激素CLCNumber:Q945DocumentCode:AArticleID:1000—3142(20l1)030370—07chinensisVar.maireiseedduringstrati
3、ficationZHANGYan-JieI,LUShun-Bao1,GAOHan-Donge(1.CollegeofLifeScience,JiangxiNormalUniversity,Nanchang330022,China;2.CollegeofForestResourcesandEnvironment,NanjingForestryUniversity,Nanjing210037,China)Abstract:InordertocharacterizerelationshipbetweenendogenousphytohormonesanddormancyofTax
4、uschinensisvar.mairei,changesincontentsoffourkindsofendogenousphytohormones,i.e.,abscisicacid(ABA),gibberellin(GA3),indoleaceticacid(IAA)andzeatinriboside(ZR)inspermodermandendospermofTaxusmaireiwereanalysedbyenzymeqinkedimmunosorbentassays(ELISA).TheresultsshowedthatABAcontentsweregradual
5、lydecreasedinendospermduringstratification,whileGAcontentswereincreasedandIAAandZRcontentsfirstlyincreasedandthendecreased.CalculatedratiodataofGA/ABA,IAA/ABAandZR/ABAexhibitedgraduallyincreasingtrendswithinstratificationtimeanddormancywasrelievedaccordinglywiththem.Inspermoderm,endogenous
6、ABA,GA,IAAandZRcontentsweregraduallydecreasedthroughstratification.ThechangeinGA/ABAratiowasgreaterthantheothertwoones.SoitwassuggestedthatthetradeoffbetweenABAandGAcontentscouldcontrolthedormancyprocessinchinensisvar.maireiseed.Keywords:Taxuscinensisvar.mairei;seed;endogenoushormonesTaz"s
7、chinensisvar.maireiSoneofrareTaxace—aeTaxusspecies,widelydistributinginChina,suchasinYangtzeRiverbasin,HenanNanlingMountains,andmountainsorvalleysinShanxi,GansuandTaiwanProvinces.Ithasattractedglobalattentionforextrac—tingsignificantanticanceractivityoftaxolfr
